Detailed information of TA system TA241236

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 821248..822083
• Replicon: chromosome
• Accession: NZ_CP095086
• Organism: Escherichia coli isolate EC5654

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A836N5X5
• Locus tag: MUK77_RS03985
• Protein ID: WP_016238532.1
• Coordinates: 821248..821625 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: MUK77_RS03990
• Protein ID: WP_016238531.1
• Coordinates: 821715..822083 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUK77_RS03950 (816274)	816274..817029	-	756	WP_000065240.1	IS21-like element ISEc10 family helper ATPase IstB	-
MUK77_RS03955 (817026)	817026..818525	-	1500	WP_016238535.1	IS21-like element ISEc10 family transposase	-
MUK77_RS03960 (818620)	818620..819162	-	543	Protein_779	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
MUK77_RS03965 (819155)	819155..819550	-	396	WP_000208383.1	DUF6088 family protein	-
MUK77_RS03970 (819619)	819619..820464	-	846	WP_001280441.1	DUF4942 domain-containing protein	-
MUK77_RS03975 (820549)	820549..820746	-	198	WP_000839260.1	DUF957 domain-containing protein	-
MUK77_RS03980 (820763)	820763..821251	-	489	WP_016238533.1	DUF5983 family protein	-
MUK77_RS03985 (821248)	821248..821625	-	378	WP_016238532.1	TA system toxin CbtA family protein	Toxin
MUK77_RS03990 (821715)	821715..822083	-	369	WP_016238531.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MUK77_RS03995 (822133)	822133..822777	-	645	WP_000094917.1	hypothetical protein	-
MUK77_RS04000 (822796)	822796..823017	-	222	WP_000692329.1	DUF987 domain-containing protein	-
MUK77_RS04005 (823080)	823080..823556	-	477	WP_001186727.1	RadC family protein	-
MUK77_RS04010 (823572)	823572..824057	-	486	WP_000849565.1	antirestriction protein	-
MUK77_RS04015 (824112)	824112..824930	-	819	WP_021533742.1	DUF932 domain-containing protein	-
MUK77_RS04020 (825031)	825031..825264	-	234	WP_001119727.1	DUF905 family protein	-
MUK77_RS04025 (825343)	825343..825798	-	456	WP_016238357.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14116.04 Da
• Isoelectric Point: 7.3842

>T241236 WP_016238532.1 NZ_CP095086:c821625-821248 [Escherichia coli]
MNTLPDTHVREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDMLRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T241236 NZ_LT963402:269215-269487 [Pseudomonas syringae pv. avii]
GTGCTAGTTGAGTGGCTTCCACAAGCTTTGGAGGCACTGGAGGAAATCGCCGGCTACCTAGCCGAGCGCAACCCTTATGC
TGCCGAGCAGATGCAGGCCACCCTTGAGGCCACGGTCGAGGCTCTCCCCCTGCACCCTTACATCCACCGTCCCGGCCGTG
TTCCAGGAACGCGCGAAGCGGTTGTGCATCCCAACTACCTGGTTGTCTACCGCGTCGGTACAGAGCGCATCAGCATCTTG
GACGTCCTGCACGCCCGCCAAGAGTACCCCTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13694.46 Da
• Isoelectric Point: 6.4768

>AT241236 WP_016238531.1 NZ_CP095086:c822083-821715 [Escherichia coli]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT241236 NZ_LT963402:269040-269225 [Pseudomonas syringae pv. avii]
ATGAGCATTCAACTTGACCCTCGCGTCTCGGAGTTCGAGACCCAGGAACAGGCCGACAATTACGACCGTTGGTTTCGCCA
GCGTATCGAGCACAGTTTGGCCGATCCGCGTCCACCCGTGCCGCACGATGAAGCAATGGCTCGCGTCAGAGCCATGATCG
AAGCAAAGCGCCGCCGTGCTAGTTGA

Structures

Toxin

• AlphaFold DB: A0A836N5X5