Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4783213..4783710 Replicon chromosome
Accession NZ_CP095083
Organism Escherichia coli strain EC6868

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MUK76_RS23435 Protein ID WP_001173343.1
Coordinates 4783213..4783386 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MUK76_RS23440 Protein ID WP_000398619.1
Coordinates 4783414..4783710 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK76_RS23425 (4779884) 4779884..4781341 + 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -
MUK76_RS23430 (4781578) 4781578..4783095 + 1518 WP_000003806.1 lysine--tRNA ligase -
MUK76_RS23435 (4783213) 4783213..4783386 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MUK76_RS23440 (4783414) 4783414..4783710 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MUK76_RS23445 (4783939) 4783939..4784211 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
MUK76_RS23450 (4784223) 4784223..4784453 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
MUK76_RS23455 (4784634) 4784634..4786265 + 1632 WP_001216477.1 sensor histidine kinase -
MUK76_RS23460 (4786262) 4786262..4786981 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - papG / papF / papE / papK / papJ / papD / papC / papH / papA / papB / papI 4733512..4783710 50198


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T241232 WP_001173343.1 NZ_CP095083:c4783386-4783213 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T241232 NZ_LT963401:9437-9712 [Pseudomonas cerasi]
ATGGAATTGAAGTGGACAAGCAAGGCGCTTTCCGACATTACCAGGCTATACGAGTTTCTGGCCTTGGCGAATCAACCTGC
CGCAGCACGAGTAGTGCAGCAACTCACCACAGCACCTACCACGCTTCTGACTAACCCGCGCATTGGCGAACGCCTAGAGG
AGTTCGAGCCAAAGGACGTACGCCGAATTCAGATCGGCCAATACGAGATGCGTTACGAGGTAGCGGATTCAACTATTTAC
CTGCTGCGCCTGTGGCACACCCGCGAAGACCGATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT241232 WP_000398619.1 NZ_CP095083:c4783710-4783414 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT241232 NZ_LT963401:9165-9437 [Pseudomonas cerasi]
ATGGCAACCACCGCCAAAACCCGATCTGTCACAGCTCACGTTCCGGTTCAGCTGGCGGAAAAAGTCGATTTGATGGCTGA
GCGTCTGGAGCGCTCTAAAAACTGGATCGTCAAGCAAGCCCTGTCTGCTTGGATAGACCAGGAAGAAGAGCGTAGTCGCC
TGACCCGTGAGGCCTTGGCTGATGTTGACGCTGGACGCGTTATTGATCACCAAGCCGTCCAGGCCTGGGCTGACAGTCTT
AGCACCGACACTCCGTTGCCGGTGCCGCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References