241223

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3848412..3849030 Replicon chromosome
Accession NZ_CP095083
Organism Escherichia coli strain EC6868

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MUK76_RS18910 Protein ID WP_001291435.1
Coordinates 3848812..3849030 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MUK76_RS18905 Protein ID WP_000344800.1
Coordinates 3848412..3848786 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK76_RS18895 (3843501) 3843501..3844694 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MUK76_RS18900 (3844717) 3844717..3847866 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
MUK76_RS18905 (3848412) 3848412..3848786 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MUK76_RS18910 (3848812) 3848812..3849030 + 219 WP_001291435.1 HHA domain-containing protein Toxin
MUK76_RS18915 (3849202) 3849202..3849753 + 552 WP_000102559.1 maltose O-acetyltransferase -
MUK76_RS18920 (3849869) 3849869..3850339 + 471 WP_000136192.1 YlaC family protein -
MUK76_RS18925 (3850503) 3850503..3852053 + 1551 WP_001389864.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MUK76_RS18930 (3852095) 3852095..3852448 - 354 WP_000878140.1 DUF1428 domain-containing protein -
MUK76_RS18940 (3852827) 3852827..3853138 + 312 WP_000409911.1 MGMT family protein -
MUK76_RS18945 (3853169) 3853169..3853741 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T241223 WP_001291435.1 NZ_CP095083:3848812-3849030 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T241223 NZ_LT963395:462556-462960 [Pseudomonas cerasi]
ATGCTCAAGTACATGCTTGACACCAATATTTGCATTTTCACTATAAAGAACAAGCCAACTTCGGTGCGTGAAGCCTTCAA
CCTCCATCATGGACAGCTATGTATCAGCGCGATCACGTTGATGGAGCTTGTCTACGGCGCAGAAAAGTCTTTGAGCCCCG
AAAGAAACCTTGCCGTTGTAGAGGGTTTCACAGCGCGTCTTGAAGTGCTCCCTTACGACAGCGACGCTGCGGCTCATACC
GGCATGATCAGAGCCGAACTGGCTAGATCGGGCACACCGATCGGCCCGTACGATCAAATGATTGCAGGACACGCCCGCTC
TCTAGGCCTGGTGGTGATCACTAATAATCAGCGTGAATTCCGACGCGTCGAGGGGCTGAGGGTAGAAGACTGGGTAAGCC
AATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT241223 WP_000344800.1 NZ_CP095083:3848412-3848786 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT241223 NZ_LT963395:462326-462556 [Pseudomonas cerasi]
ATGGAACAATCCACAGTCTTCAAAAGCAATCGAAGCCAGGCTGTCCGACTGCCAAAAGCGGTCGCCTTGCCAAACGACGT
GAAACGTGTCGATGTCGTAGCCGTGGGCAGAACCCGCATTATTTCCCCCGCTGGTGAAATGTGGAACAGCTGGTTCGATG
GTGAAAGTGTCAGTGATGATTTCATGGCAGAGCGTGAACAACCAGCCGAACAGCAGCGCGAATCGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References