Detailed information of TA system TA241222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3355485..3356190
• Replicon: chromosome
• Accession: NZ_CP095083
• Organism: Escherichia coli strain EC6868

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: MUK76_RS16460
• Protein ID: WP_000539521.1
• Coordinates: 3355485..3355871 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MUK76_RS16465
• Protein ID: WP_001280945.1
• Coordinates: 3355861..3356190 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUK76_RS16440 (3351489)	3351489..3352115	+	627	WP_001295292.1	glutathione S-transferase GstB	-
MUK76_RS16445 (3352112)	3352112..3353227	-	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
MUK76_RS16450 (3353338)	3353338..3353721	-	384	WP_000497137.1	biofilm formation regulator BssR	-
MUK76_RS16455 (3353934)	3353934..3355259	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
MUK76_RS16460 (3355485)	3355485..3355871	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MUK76_RS16465 (3355861)	3355861..3356190	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
MUK76_RS16470 (3356260)	3356260..3357588	-	1329	WP_000086879.1	GGDEF domain-containing protein	-
MUK76_RS16475 (3357596)	3357596..3359944	-	2349	WP_001322378.1	EAL domain-containing protein	-
MUK76_RS16480 (3360122)	3360122..3361033	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T241222 WP_000539521.1 NZ_CP095083:3355485-3355871 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T241222 NZ_LT963394:c105779-105510 [Pseudomonas syringae pv. cerasicola]
ATGCTGGTTGAGTGGCGGCCCGAAGCACGTGCCGAACTCTGGAGCATTTTGAACTACATCACTGATCAAAATCCGATGGC
AGCCGAGAGGCTGAACCAGACTATTGAGGCGTCTACAACGGCCCTTCCACAACACCCTTATCTGTACCGATTAGGGCAAG
TTTATGGTACTCGGGAAATGGTCGTACACCCCAATTACGTAGTGGTTTACCGGGTGACTGATCGAATTGAAATCGTCAAC
GTCCTCCACGCCCGTCAGCAATACCCTTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT241222 WP_001280945.1 NZ_CP095083:3355861-3356190 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT241222 NZ_LT963394:c105960-105769 [Pseudomonas syringae pv. cerasicola]
ATGAGCACCCAACTTTCCCCCATCGTTTCCGAGTTCGAAACACAGGAACAAGCAGACAGCTATGACAGGTGGTTTCGGGC
CAAGGTCCAAGCATCACTCGATGACCCACGTCCCAACATCCCCCACGATCAGGTGATGTCTGAAATGCGTGCACTCATCG
AGTCCAAGAAGAATAAGCATAATGCTGGTTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6