Detailed information of TA system TA241221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3105761..3106545
• Replicon: chromosome
• Accession: NZ_CP095083
• Organism: Escherichia coli strain EC6868

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: MUK76_RS15275
• Protein ID: WP_000613626.1
• Coordinates: 3106051..3106545 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: MUK76_RS15270
• Protein ID: WP_001110447.1
• Coordinates: 3105761..3106054 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUK76_RS15260 (3100911)	3100911..3101870	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
MUK76_RS15265 (3102443)	3102443..3105628	+	3186	WP_000827427.1	ribonuclease E	-
MUK76_RS15270 (3105761)	3105761..3106054	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
MUK76_RS15275 (3106051)	3106051..3106545	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
MUK76_RS15280 (3106640)	3106640..3107593	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
MUK76_RS15285 (3107605)	3107605..3109248	-	1644	WP_000096524.1	flagellar hook-associated protein FlgK	-
MUK76_RS15290 (3109314)	3109314..3110255	-	942	WP_001309406.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
MUK76_RS15295 (3110255)	3110255..3111352	-	1098	WP_000589320.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T241221 WP_000613626.1 NZ_CP095083:3106051-3106545 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T241221 NZ_LT963394:c4987-4610 [Pseudomonas syringae pv. cerasicola]
GTGAAAGGCGTCCTGGTAGACACCTCCGTTTGGGTTGAGCACTTTCGCAACAACAGTCCGGAACTGGTGAACTTGCTCAG
CCAGGACCGGGTACTCATACACCCCATGGTGATCGGAGAACTGGCGTGTGGTACGCCGCCTGATCGTTCGAACACCTTGA
CCGACCTTGGCGACCTACGAGGTGCACAACAACCCACGGTGAGTGAAGTCATCGCCTTTCTGAACACCCACAAGCTCTAT
GGCCTGGGCTGTGGGCTGGTGGATATGACGCTCCTGGCCTCCGCGTTATTGAGCGGCACAGCGCTTTGGACGTTAGACAA
GCGCCTGGAGCGTTTGGCTAGCCGCATGGCCGTGTCATACCAGCCTCCTACTCACTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT241221 WP_001110447.1 NZ_CP095083:3105761-3106054 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT241221 NZ_LT963394:c5184-4984 [Pseudomonas syringae pv. cerasicola]
ATGAGAACGACCGTCACCATCGACGATGCGTTGTACCAACGTGCTCTGGAAGTCGCCGATCCGGCGATGGATAAGGCCGA
CCTGTTCCGTGAAGCCGTTCAGACGTTTGTGCGCATTCAAGCGGCTAAACGATTGATGGCTTTAGGGGCCACACTGCCAG
CGATGGAAGACATCGCCCGCCGACACGAGAAGGCACTGTGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6