241220

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2656140..2656778 Replicon chromosome
Accession NZ_CP095083
Organism Escherichia coli strain EC6868

Toxin (Protein)


Gene name hicA Uniprot ID B7N4J4
Locus tag MUK76_RS12715 Protein ID WP_000813797.1
Coordinates 2656602..2656778 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag MUK76_RS12710 Protein ID WP_076611057.1
Coordinates 2656140..2656556 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK76_RS12690 (2651292) 2651292..2652233 - 942 WP_001251335.1 ABC transporter permease -
MUK76_RS12695 (2652234) 2652234..2653247 - 1014 WP_000220413.1 ABC transporter ATP-binding protein -
MUK76_RS12700 (2653265) 2653265..2654410 - 1146 WP_116842935.1 ABC transporter substrate-binding protein -
MUK76_RS12705 (2654655) 2654655..2656061 - 1407 WP_000760613.1 PLP-dependent aminotransferase family protein -
MUK76_RS12710 (2656140) 2656140..2656556 - 417 WP_076611057.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
MUK76_RS12715 (2656602) 2656602..2656778 - 177 WP_000813797.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
MUK76_RS12720 (2657000) 2657000..2657230 + 231 WP_000494244.1 YncJ family protein -
MUK76_RS12725 (2657322) 2657322..2659283 - 1962 WP_001309488.1 23S rRNA 5-hydroxycytidine C2501 synthase -
MUK76_RS12730 (2659356) 2659356..2659892 - 537 WP_000429161.1 DNA-binding transcriptional regulator SutR -
MUK76_RS12735 (2659945) 2659945..2661156 + 1212 WP_001323162.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6724.76 Da        Isoelectric Point: 11.5666

>T241220 WP_000813797.1 NZ_CP095083:c2656778-2656602 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T241220 NZ_LT963393:c72036-71755 [Pseudomonas syringae pv. cerasicola]
ATGCTGCCGGTGGTCTGGCTCAGTCCCGCACTGGATGATCTGCGCGAAATCGCAACCTACATCGCATGGGAAAACCCGAG
CGCAGCGCGCCGGTTGAAAAGCCTCCTGCAGGAGGCTATCGAGCCGGTAGCCGAACACCCCTATTTATATCGTTCTGGGC
GAGCGCCAGGCACTCGCGAGCTGGTGGCTCACCCAAACTACGTCCTGGTCTATCGTGTCACCCTCGAGCGCATCGAGGTC
GTGAACGTAATCCACGCCAGGCAGGAATACCCCTCTCCCTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15251.65 Da        Isoelectric Point: 4.5908

>AT241220 WP_076611057.1 NZ_CP095083:c2656556-2656140 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT241220 NZ_LT963393:c72224-72036 [Pseudomonas syringae pv. cerasicola]
ATGGCCAACCTCTCACCCATCGTTTCAGAATTCGAAACCGATGAACAGGCCGCAAGCTACGACCGCTGGTTTCGCGCCAA
GGTCGCCGCCAGTCTGGCCGATCCGCGTCCGGCCATCCCTCACGATGAGGTCATGGCGGAAATAGAAGCGCTGATCGATC
AGGTTGAAACCCAACGTGAGAAACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J715

Antitoxin

Source ID Structure

References