Detailed information of TA system TA241218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2555628..2557216
• Replicon: chromosome
• Accession: NZ_CP095083
• Organism: Escherichia coli strain EC6868

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9YVT8
• Locus tag: MUK76_RS12270
• Protein ID: WP_001125415.1
• Coordinates: 2555894..2557216 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: MUK76_RS12265
• Protein ID: WP_001296726.1
• Coordinates: 2555628..2555894 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUK76_RS12265 (2555628)	2555628..2555894	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
MUK76_RS12270 (2555894)	2555894..2557216	+	1323	WP_001125415.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
MUK76_RS12275 (2557614)	2557614..2558513	-	900	WP_001057776.1	LysR family transcriptional regulator	-
MUK76_RS12280 (2558653)	2558653..2558961	+	309	WP_001221046.1	hypothetical protein	-
MUK76_RS12285 (2558970)	2558970..2559659	+	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
MUK76_RS12290 (2559733)	2559733..2560680	+	948	WP_000854796.1	aldo/keto reductase	-
MUK76_RS12295 (2560783)	2560783..2560938	+	156	WP_001322415.1	hypothetical protein	-
MUK76_RS12300 (2560898)	2560898..2561317	+	420	WP_001322416.1	amidohydrolase family protein	-
MUK76_RS26595 (2561307)	2561307..2561459	+	153	WP_001322417.1	amidohydrolase family protein	-
MUK76_RS12305 (2561610)	2561610..2562182	+	573	WP_000789913.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49384.05 Da
• Isoelectric Point: 8.5830

>T241218 WP_001125415.1 NZ_CP095083:2555894-2557216 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK

Nucleotide

• Length: 1323 bp

>T241218 NZ_LT963350:1044586-1044831 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT241218 WP_001296726.1 NZ_CP095083:2555628-2555894 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT241218 NZ_LT963350:1044831-1045145 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: U9YVT8