Detailed information of TA system TA241204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 150199..150811
• Replicon: chromosome
• Accession: NZ_CP095083
• Organism: Escherichia coli strain EC6868

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: MUK76_RS00645
• Protein ID: WP_000833473.1
• Coordinates: 150199..150384 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: MUK76_RS00650
• Protein ID: WP_000499744.1
• Coordinates: 150401..150811 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUK76_RS00635 (147031)	147031..148182	+	1152	WP_000741501.1	L-threonine dehydrogenase	-
MUK76_RS00640 (148383)	148383..149921	+	1539	WP_001309845.1	aldehyde dehydrogenase AldB	-
MUK76_RS00645 (150199)	150199..150384	+	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
MUK76_RS00650 (150401)	150401..150811	+	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
MUK76_RS00655 (150883)	150883..152847	-	1965	WP_001026861.1	glycoside hydrolase family 127 protein	-
MUK76_RS00660 (152858)	152858..154258	-	1401	WP_000204802.1	MFS transporter	-
MUK76_RS00665 (154484)	154484..155299	+	816	WP_000891825.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T241204 WP_000833473.1 NZ_CP095083:150199-150384 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T241204 NZ_LT962928:1044622-1044867 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT241204 WP_000499744.1 NZ_CP095083:150401-150811 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT241204 NZ_LT962928:1044867-1045181 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9