Detailed information of TA system TA024120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1479396..1480034
• Replicon: chromosome
• Accession: NC_012967
• Organism: Escherichia coli B str. REL606

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ECB_RS07405
• Protein ID: WP_000813794.1
• Coordinates: 1479396..1479572 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ECB_RS07410
• Protein ID: WP_001270286.1
• Coordinates: 1479618..1480034 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECB_RS07385	1475015..1476229	-	1215	WP_001326689.1	BenE family transporter YdcO	-
ECB_RS07390	1476282..1476818	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
ECB_RS07395	1476891..1478852	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ECB_RS07400	1478944..1479174	-	231	WP_000494237.1	YncJ family protein	-
ECB_RS07405	1479396..1479572	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ECB_RS07410	1479618..1480034	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ECB_RS07415	1480113..1481519	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
ECB_RS07420	1481764..1482909	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
ECB_RS07425	1482927..1483940	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
ECB_RS07430	1483941..1484882	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T24120 WP_000813794.1 NC_012967:1479396-1479572 [Escherichia coli B str. REL606]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T24120 NC_012967:1479396-1479572 [Escherichia coli B str. REL606]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT24120 WP_001270286.1 NC_012967:1479618-1480034 [Escherichia coli B str. REL606]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT24120 NC_012967:1479618-1480034 [Escherichia coli B str. REL606]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB