Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 621392..622027 Replicon chromosome
Accession NZ_CP095072
Organism Gracilibacillus sp. SSWR10-1

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag MUN88_RS03025 Protein ID WP_244720658.1
Coordinates 621392..621742 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag MUN88_RS03030 Protein ID WP_244720661.1
Coordinates 621746..622027 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUN88_RS02990 (MUN88_02990) 616803..617594 - 792 WP_244720644.1 RNA polymerase sigma factor SigB -
MUN88_RS02995 (MUN88_02995) 617566..618042 - 477 WP_244720645.1 anti-sigma B factor RsbW -
MUN88_RS03000 (MUN88_03000) 618043..618372 - 330 WP_244720646.1 STAS domain-containing protein -
MUN88_RS03005 (MUN88_03005) 618489..619493 - 1005 WP_244720648.1 PP2C family protein-serine/threonine phosphatase -
MUN88_RS03010 (MUN88_03010) 619587..619988 - 402 WP_244720650.1 anti-sigma regulatory factor -
MUN88_RS03015 (MUN88_03015) 619991..620347 - 357 WP_244720652.1 STAS domain-containing protein -
MUN88_RS03020 (MUN88_03020) 620361..621224 - 864 WP_244720655.1 RsbT co-antagonist protein RsbRA -
MUN88_RS03025 (MUN88_03025) 621392..621742 - 351 WP_244720658.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MUN88_RS03030 (MUN88_03030) 621746..622027 - 282 WP_244720661.1 antitoxin Antitoxin
MUN88_RS03035 (MUN88_03035) 622204..623322 - 1119 WP_244720665.1 alanine racemase -
MUN88_RS03040 (MUN88_03040) 624338..625141 - 804 WP_244720668.1 hypothetical protein -
MUN88_RS03045 (MUN88_03045) 625299..626279 - 981 WP_244720671.1 sugar phosphate nucleotidyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12969.96 Da        Isoelectric Point: 5.1634

>T241199 WP_244720658.1 NZ_CP095072:c621742-621392 [Gracilibacillus sp. SSWR10-1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVLQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAEKYGFERNSVILLEQI
RTIDKQRLTDKITQLDSNMMERIDRGLEISLGLTDF

Download         Length: 351 bp

>T241199 NZ_LT962918:1044766-1045011 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCTTTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.07 Da        Isoelectric Point: 4.4508

>AT241199 WP_244720661.1 NZ_CP095072:c622027-621746 [Gracilibacillus sp. SSWR10-1]
MVLSEDLQEVAVRLPKNLLNEVDGLMKSEDSNLSDFICQATRTYLRETKKRHIQESMQRGYMEMAKINLNIASEAFQAEE
EAENTLERLVSGV

Download         Length: 282 bp

>AT241199 NZ_LT962918:1045011-1045325 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References