Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2868255..2868892 Replicon chromosome
Accession NZ_CP095023
Organism Mycobacterium tuberculosis strain SGF0702019

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag MUW68_RS13455 Protein ID WP_003413180.1
Coordinates 2868255..2868650 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag MUW68_RS13460 Protein ID WP_003413183.1
Coordinates 2868647..2868892 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW68_RS13415 (MUW68_13415) 2863659..2864870 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
MUW68_RS13420 (MUW68_13420) 2864997..2865656 + 660 WP_003902296.1 LppA family lipoprotein -
MUW68_RS13425 (MUW68_13425) 2865653..2866315 + 663 WP_003905878.1 LppA family lipoprotein -
MUW68_RS13430 (MUW68_13430) 2866312..2866589 + 278 Protein_2655 type II toxin-antitoxin system VapB family antitoxin -
MUW68_RS13435 (MUW68_13435) 2866682..2867095 + 414 WP_003413164.1 PIN domain nuclease -
MUW68_RS13440 (MUW68_13440) 2867134..2867391 + 258 WP_003413167.1 CopG family transcriptional regulator -
MUW68_RS13445 (MUW68_13445) 2867388..2867765 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
MUW68_RS13450 (MUW68_13450) 2867781..2868155 - 375 WP_003413177.1 hypothetical protein -
MUW68_RS13455 (MUW68_13455) 2868255..2868650 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
MUW68_RS13460 (MUW68_13460) 2868647..2868892 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
MUW68_RS13465 (MUW68_13465) 2869303..2869722 - 420 WP_003413190.1 A24 family peptidase -
MUW68_RS13470 (MUW68_13470) 2869734..2870543 - 810 WP_003413193.1 shikimate dehydrogenase -
MUW68_RS13475 (MUW68_13475) 2870540..2871793 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
MUW68_RS13480 (MUW68_13480) 2871786..2872298 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T241175 WP_003413180.1 NZ_CP095023:c2868650-2868255 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T241175 NZ_LT960611:c3669715-3669401 [Vibrio tapetis subsp. tapetis]
ATGCACGTAATAAGTAAAAGACCATTTAATGATGCTAAGAAAAATTTTCCTACGTGTGCCGAAGCTTTGGATGCAGCATA
TAAAGTATTGCGTATAAGTCGAGCTAAAACACCGGAGGAATTAAAAGCTTTGTTTCCATCATTAGATAATTGTAAGTACA
GGGATAAGTTGTATGTTATTGACATTGGCGGTAACAAATTGCGATTGATGGCTTACATTGAATTTACGCAAGGGCGATTT
TACGTAAAGCATATTGTTACTCATGCGGAGTATGACAAGATAATTAAATCTTATCGAAGTGAGAAGAAAAAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT241175 WP_003413183.1 NZ_CP095023:c2868892-2868647 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT241175 NZ_LT960611:c3669392-3668976 [Vibrio tapetis subsp. tapetis]
ATGAATATTACACGAATGAAAGAGTTTTCTAACCAAATGATTGAAGTTATGCCTTGGATTAATGGTATCTCTACTTCTGA
TGAATACGACCAACTCATCGAGTTAATGGATGAGTTGGTCGAAGACTACGAAGGAAATCAAGTGCTTATTGACCTTTTAT
TTCCCGTTATCGAAAGATATGAATCTGAATCTGAAGAGTTCAATGAATTCAACCGTGTTGTTGGTGAGTTAGAGCCTGGG
TTAGCAATGCTCAGAGTGATCATTGATCAAAATGAAATGACTCTATCAGATTTTAAAGATGAAATTGGAGCAAAATCTAC
AGTATCAATGATTCTGAGTGGTTCACGTTCTTTGACTCTCCGACATATTAAATCCTTGTCGGAACGCTTTAATATTCCAG
CCTATATGTTCATCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References