Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2867134..2867765 Replicon chromosome
Accession NZ_CP095023
Organism Mycobacterium tuberculosis strain SGF0702019

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag MUW68_RS13445 Protein ID WP_003413174.1
Coordinates 2867388..2867765 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag MUW68_RS13440 Protein ID WP_003413167.1
Coordinates 2867134..2867391 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW68_RS13410 (MUW68_13410) 2863049..2863363 + 315 WP_009937839.1 hypothetical protein -
MUW68_RS13415 (MUW68_13415) 2863659..2864870 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
MUW68_RS13420 (MUW68_13420) 2864997..2865656 + 660 WP_003902296.1 LppA family lipoprotein -
MUW68_RS13425 (MUW68_13425) 2865653..2866315 + 663 WP_003905878.1 LppA family lipoprotein -
MUW68_RS13430 (MUW68_13430) 2866312..2866589 + 278 Protein_2655 type II toxin-antitoxin system VapB family antitoxin -
MUW68_RS13435 (MUW68_13435) 2866682..2867095 + 414 WP_003413164.1 PIN domain nuclease -
MUW68_RS13440 (MUW68_13440) 2867134..2867391 + 258 WP_003413167.1 CopG family transcriptional regulator Antitoxin
MUW68_RS13445 (MUW68_13445) 2867388..2867765 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
MUW68_RS13450 (MUW68_13450) 2867781..2868155 - 375 WP_003413177.1 hypothetical protein -
MUW68_RS13455 (MUW68_13455) 2868255..2868650 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
MUW68_RS13460 (MUW68_13460) 2868647..2868892 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
MUW68_RS13465 (MUW68_13465) 2869303..2869722 - 420 WP_003413190.1 A24 family peptidase -
MUW68_RS13470 (MUW68_13470) 2869734..2870543 - 810 WP_003413193.1 shikimate dehydrogenase -
MUW68_RS13475 (MUW68_13475) 2870540..2871793 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
MUW68_RS13480 (MUW68_13480) 2871786..2872298 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T241174 WP_003413174.1 NZ_CP095023:2867388-2867765 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T241174 NZ_LT960611:3653365-3654306 [Vibrio tapetis subsp. tapetis]
ATGAAAAAACTAAAACAACAATTCCTTGAGGTTTCCGATGCTTTAGCGCTAGGAAACCCAGCCATTATTGAAAAAGATTA
CTGGGTTGTTGCTCTGCTTGCTGAGCTAGAAAAACTTACCTTTGAAACTCACCAATTGGTTTTTGCAGGTGGTACGGCAT
TAGCCAAATCTAATGTCAAAATCCTACGGATGTCAGAAGACGTTGATATTAAACTGATCCCAAGTAAAGAGTTTGATGCG
TTGAGTCGTGGTAAAAAGAAAACCGCACGTAAAATGCTTATCGAGCAAATAGAGCAGGTGATTGCGCGATCTCAGCAATT
TACAGTTGAAAGCCGACTTGTTCGTGATGAATACCGTTATGTGGAGTATGAACTTAAATATCCTCAACAATTCAACCAAG
CTCCATGCTTGCGACCAATCATCAAATTAGAGCTGATGGAAACTATTCCGCTTCTAGAAGTAGAAGACCGAGGAATTCAA
TCGTTAGTTGCAGAGCTTTATAAGCAACCACAAGAGGTCAGCGCATTCGCTTGTGTTTCTGTTTACGCAACATTAGTGGA
GAAAGTACTTTCTATGTTACGTCGTACTATGTTGGTTAAGCGTGACACCGAACGTAAAGATGATGTCACGTTGGTCCGTC
ATATCTACGACGTTCATTGCATTGATACAGCGCAGACGCTAGATATTGAGTCACTAACCTCATTATTTCTCACAGTATTG
AAAGAAGATATCGACCGCTTTGGTAACCAGCATGCTGAGTTTGTCGCTGCCCCTATTAAGGAGCTACGCCTTGGCTTACA
AGAGCTAGAAGACAACCCGATTTTCCGCCAACGCTTTCAAGACTTTGTTACGCCAATGGTGTTTAACACCGAACCCCATG
ATTTTGATACCTGCTTTGCCTCATTTAAACGCATCGCTGAATCATTGATTAAAACAATTTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT241174 WP_003413167.1 NZ_CP095023:2867134-2867391 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT241174 NZ_LT960611:3652958-3653362 [Vibrio tapetis subsp. tapetis]
ATGACTGCGGCAGATCGTATTTATCAGAATATAAAGCGTTCAAGACGCTATGTCTTTGAACGCAAAGACTTTGATGGGTT
TGCCAGTTATGATCAAGTAGGTAGAGCATTGAGGCAATTAGTAAAGCAAGGTGAACTAATGAAGCTTGGCTATGGTTTGT
ATACCAAAGCAAGGGTTAATAGCCTTACAGGAAGACCCATGCCTACCAACCCTGGTGGTAGCGATGCCCTTATCCGGGAA
ATATTAAAAATGAAGGGCGTTGACTTTGAGATGGATAGTTTGTCTCTACAGAGCTTTTCAGGTGAAAGCACACAAGTACC
ATCATCCGTTCATTATTCATGGAATCCTAAGCAGTTTAATCGCAAGCTGGTAGTAGGTAATCGAGTACTGAATTCTCCGA
GTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References