Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2322956..2323592 Replicon chromosome
Accession NZ_CP095023
Organism Mycobacterium tuberculosis strain SGF0702019

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag MUW68_RS10850 Protein ID WP_003410654.1
Coordinates 2323182..2323592 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag MUW68_RS10845 Protein ID WP_003410651.1
Coordinates 2322956..2323189 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW68_RS10830 (MUW68_10830) 2318404..2318805 + 402 WP_009937711.1 metal ABC transporter permease -
MUW68_RS10835 (MUW68_10835) 2318806..2319210 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
MUW68_RS10840 (MUW68_10840) 2319294..2322878 - 3585 WP_003410648.1 cobaltochelatase subunit CobN -
MUW68_RS10845 (MUW68_10845) 2322956..2323189 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
MUW68_RS10850 (MUW68_10850) 2323182..2323592 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
MUW68_RS10855 (MUW68_10855) 2323576..2324667 + 1092 WP_003900454.1 precorrin-3B synthase -
MUW68_RS10860 (MUW68_10860) 2324677..2325303 + 627 WP_003410658.1 precorrin-8X methylmutase -
MUW68_RS10865 (MUW68_10865) 2325300..2326826 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
MUW68_RS10870 (MUW68_10870) 2326772..2327995 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T241168 WP_003410654.1 NZ_CP095023:2323182-2323592 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T241168 NZ_LT934425:3488847-3489140 [Candidatus Kuenenia stuttgartiensis]
GTGATACAATCATTCCATGATAAGGGGACAGAGGATATTTTTGATAGAAAAAACACCAGAGAGGCGCGTAAGACCTGTCC
GCAACAAATCTGGCGAGCGGCGCAACGAAAGCTCGATCAACTTAATGGAGTTGTTTCTTTAGGTTCGCTGAAAATTCCAC
CGGGAAACATGCTGGAAGTTCTTAAAGATGATCGAAAGGGACAGCACAGTATACGGATTAACGATCAGTTCCGAGTTTGT
TTTGTGTGGGCTGATGATGGTGTAGATAACGTCGAAATTACAGATTACCACTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT241168 WP_003410651.1 NZ_CP095023:2322956-2323189 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT241168 NZ_LT934425:3489159-3489476 [Candidatus Kuenenia stuttgartiensis]
ATGGTTCGTATACCAAAAAATGGTCCGCCTACGCATCCGGGCGAAATGTTATTGGAGGAATTTCTCAAACCTCTGCATAT
GACGCAGCGCGAGTTGGCAGAAAAGCTTGGGGTTTCATATCCGCGTGTTAATGAACTGATTCATGGAAAACGCGGGATGA
CTCCAGACACTGCGCTTCGTCTGGAGAAACTTTTCGGCATGGACGCACAGTTTTGGCTTAATTTGCAGTTGGCATGGGAT
CTCTATCATGTAACCCACTCATCAACTGCGAAGGAGATACAAAAGATAAAAAGATTGCCTGCTTTTATCCATGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References