Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2252455..2253081 Replicon chromosome
Accession NZ_CP095023
Organism Mycobacterium tuberculosis strain SGF0702019

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag MUW68_RS10550 Protein ID WP_003410075.1
Coordinates 2252683..2253081 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A806JLL8
Locus tag MUW68_RS10545 Protein ID WP_003911750.1
Coordinates 2252455..2252682 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW68_RS10535 (MUW68_10535) 2250494..2250838 - 345 WP_003410065.1 ferredoxin family protein -
MUW68_RS10540 (MUW68_10540) 2251027..2252196 - 1170 WP_003899126.1 ATP-binding protein -
MUW68_RS10545 (MUW68_10545) 2252455..2252682 + 228 WP_003911750.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MUW68_RS10550 (MUW68_10550) 2252683..2253081 + 399 WP_003410075.1 PIN domain nuclease Toxin
MUW68_RS10555 (MUW68_10555) 2253264..2253695 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
MUW68_RS10560 (MUW68_10560) 2253796..2254230 + 435 WP_003914358.1 DUF1398 domain-containing protein -
MUW68_RS10565 (MUW68_10565) 2254667..2254846 - 180 Protein_2090 hypothetical protein -
MUW68_RS10570 (MUW68_10570) 2254934..2255554 + 621 WP_003410086.1 IS110 family transposase -
MUW68_RS10575 (MUW68_10575) 2255508..2256098 + 591 WP_003899131.1 IS110 family transposase -
MUW68_RS10580 (MUW68_10580) 2256226..2257482 - 1257 WP_003902247.1 HNH endonuclease signature motif containing protein -
MUW68_RS10585 (MUW68_10585) 2257836..2258036 + 201 Protein_2094 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T241166 WP_003410075.1 NZ_CP095023:2252683-2253081 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T241166 NZ_LT934425:c2021000-2020740 [Candidatus Kuenenia stuttgartiensis]
ATGAGTAAGGCTGAGAAATTATTGAGGCGTTTCTTATCAAAGCCAAAGGATTTCACCTATGATGATCTTAAAAGTCTTCT
GGCTGGATTTGGTTATGAAAAGGCAAAGTCAGGCAAAACTACCGGGTCGGGGGTAACCTTTATTAATCCGGAAACAAGGC
ATATTATAAGATTGCATAAACCACATCCGGGAGTTGAGCTTAAGAGATATCAATTGGATTATATAGAAGAAGCATTAAGA
AATAAGGGGTTAATACAATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8346.16 Da        Isoelectric Point: 4.1621

>AT241166 WP_003911750.1 NZ_CP095023:2252455-2252682 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 228 bp

>AT241166 NZ_LT934425:c2020743-2020405 [Candidatus Kuenenia stuttgartiensis]
ATGAAGGATGTTTTAACGTACAAAGGTTTCACGGGAACTGTGCATTTCAGCGCAGAGGATAAAGTTTTTCATGGGAAAAT
CGAGGGTATAGATGACCTTGTAATCTTTGAGGGTCATAGTGTAGAAGAACTAGTAAAGGCATTTCACAATGCGGCGGATG
ATTATGCTGCTCTTTGCAGAGAAATGGGTAAAGTGCCTATAAAGTCATATAGGGGAAGTTTTAATGTTCGTATACCGCCA
GAGATGCACAGAAAAGCAGCCGAGAGAGCTATTCAAAAAGGCTTGTCATTAAATCAGCTTGTTCAAACAGCAATAGAGAA
AGAATTGGTACATAATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
AlphaFold DB A0A806JLL8

References