Detailed information of TA system TA241151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1241377..1241945
• Replicon: chromosome
• Accession: NZ_CP095023
• Organism: Mycobacterium tuberculosis strain SGF0702019

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: MUW68_RS05940
• Protein ID: WP_003405865.1
• Coordinates: 1241571..1241945 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: MUW68_RS05935
• Protein ID: WP_003405863.1
• Coordinates: 1241377..1241574 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW68_RS05915 (MUW68_05915)	1237418..1238056	-	639	WP_003898730.1	lipid droplet-associated protein	-
MUW68_RS05920 (MUW68_05920)	1238146..1239153	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
MUW68_RS05925 (MUW68_05925)	1239170..1240153	-	984	WP_003898731.1	hypothetical protein	-
MUW68_RS05930 (MUW68_05930)	1240216..1241289	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
MUW68_RS05935 (MUW68_05935)	1241377..1241574	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MUW68_RS05940 (MUW68_05940)	1241571..1241945	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUW68_RS05945 (MUW68_05945)	1242148..1242846	+	699	WP_244733051.1	C40 family peptidase	-
MUW68_RS05950 (MUW68_05950)	1242964..1243149	+	186	WP_003901093.1	hypothetical protein	-
MUW68_RS05955 (MUW68_05955)	1243076..1243351	-	276	WP_003405867.1	hypothetical protein	-
MUW68_RS05960 (MUW68_05960)	1243594..1243917	+	324	WP_003405871.1	putative quinol monooxygenase	-
MUW68_RS05965 (MUW68_05965)	1243932..1244630	-	699	WP_031646953.1	hypothetical protein	-
MUW68_RS05970 (MUW68_05970)	1244664..1244873	+	210	WP_003911400.1	hypothetical protein	-
MUW68_RS05975 (MUW68_05975)	1244825..1245595	-	771	Protein_1178	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T241151 WP_003405865.1 NZ_CP095023:1241571-1241945 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T241151 NZ_LT907990:c343517-343239 [Vibrio cholerae]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT241151 WP_003405863.1 NZ_CP095023:1241377-1241574 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT241151 NZ_LT907990:c343798-343514 [Vibrio cholerae]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07