Detailed information of TA system TA241148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1016555..1017174
• Replicon: chromosome
• Accession: NZ_CP095023
• Organism: Mycobacterium tuberculosis strain SGF0702019

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MUW68_RS04810
• Protein ID: WP_003404726.1
• Coordinates: 1016740..1017174 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: MUW68_RS04805
• Protein ID: WP_003898641.1
• Coordinates: 1016555..1016734 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW68_RS04790 (MUW68_04790)	1012028..1013608	+	1581	WP_003910977.1	serine hydrolase	-
MUW68_RS04795 (MUW68_04795)	1013605..1015998	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
MUW68_RS04800 (MUW68_04800)	1016271..1016429	+	159	WP_003404720.1	hypothetical protein	-
MUW68_RS04805 (MUW68_04805)	1016555..1016734	+	180	WP_003898641.1	antitoxin	Antitoxin
MUW68_RS04810 (MUW68_04810)	1016740..1017174	+	435	WP_003404726.1	SRPBCC family protein	Toxin
MUW68_RS04815 (MUW68_04815)	1017272..1018045	+	774	WP_003404735.1	VOC family protein	-
MUW68_RS04820 (MUW68_04820)	1018110..1018559	+	450	WP_003404738.1	hypothetical protein	-
MUW68_RS04825 (MUW68_04825)	1018694..1018924	-	231	WP_003898642.1	hypothetical protein	-
MUW68_RS04830 (MUW68_04830)	1019091..1020599	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
MUW68_RS04835 (MUW68_04835)	1020601..1021839	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T241148 WP_003404726.1 NZ_CP095023:1016740-1017174 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T241148 NZ_LT907990:c326383-325886 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT241148 WP_003898641.1 NZ_CP095023:1016555-1016734 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT241148 NZ_LT907990:c326652-326380 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5