Detailed information of TA system TA241139

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 704544..705187
• Replicon: chromosome
• Accession: NZ_CP095023
• Organism: Mycobacterium tuberculosis strain SGF0702019

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: MUW68_RS03185
• Protein ID: WP_003403187.1
• Coordinates: 704786..705187 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: MUW68_RS03180
• Protein ID: WP_003403184.1
• Coordinates: 704544..704789 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW68_RS03145 (MUW68_03145)	699839..700369	-	531	WP_003917320.1	two component system sensor kinase HK2	-
MUW68_RS03150 (MUW68_03150)	700353..701033	-	681	WP_244733088.1	two-component system response regulator TcrA	-
MUW68_RS03155 (MUW68_03155)	701156..701467	+	312	WP_003403164.1	hypothetical protein	-
MUW68_RS03160 (MUW68_03160)	701539..702489	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
MUW68_RS03165 (MUW68_03165)	702730..703314	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
MUW68_RS03170 (MUW68_03170)	703316..704026	+	711	Protein_629	IS607 family element RNA-guided endonuclease TnpB	-
MUW68_RS03175 (MUW68_03175)	704029..704499	+	471	WP_003898523.1	hypothetical protein	-
MUW68_RS03180 (MUW68_03180)	704544..704789	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MUW68_RS03185 (MUW68_03185)	704786..705187	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUW68_RS03190 (MUW68_03190)	705178..705357	+	180	Protein_633	hypothetical protein	-
MUW68_RS03195 (MUW68_03195)	705775..705972	-	198	WP_003403191.1	hypothetical protein	-
MUW68_RS03200 (MUW68_03200)	706052..707209	-	1158	WP_003898524.1	hypothetical protein	-
MUW68_RS03205 (MUW68_03205)	707261..707482	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
MUW68_RS03210 (MUW68_03210)	707624..708229	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T241139 WP_003403187.1 NZ_CP095023:704786-705187 [Mycobacterium tuberculosis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T241139 NZ_LT907988:c913803-913507 [Orrella dioscoreae]
ATGACAAGGCCGACACTGCGGGAATGGGACAGCGCGGATCATCTCGGCACGGAGAAGGACCGCGTGCTGTATCTGCAGGC
CTGCATGGAAGAGGCAGGCGACGATGCCACCTTCATTGCCAAGGCCCTGGGCGACATTGCCCGCGCCAAAGGGATGACGC
AATTGGCACGAGACACAGGCCTGGGCCGGGAAAGCCTCTACAAGGCGCTGTCAGGCGAGGGAAATCCGAGTTTTGCCACC
ATATTGAAAGTGACCCGGGCGCTGGGCATCAGGCTGCAACCGCAACTGCAGGCCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT241139 WP_003403184.1 NZ_CP095023:704544-704789 [Mycobacterium tuberculosis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT241139 NZ_LT907988:c914105-913806 [Orrella dioscoreae]
TTGATCCGCGTTTACACCACCCCCACATTTGATCGCTGGTTCGCACAGATCGACGACACGCTGACAGCCAAACGCATCCA
AGTCCGGATTCGCCGCCTGCAGCTCGGCAACCACGGAGACTGGGCGCCAATCGGTGAAGGTGTCGCGGAACTGCGGATCC
ATCATGGCCCCGGATACCGCGTGGTTTTCATGCCGCGAACCACGGACATCATCGTGCTGCTGGCAGGGGGCGACAAATCC
ACCCAGCCGCGGGACATCCGCGCCGCCCTGTCGCTGGCGCGCCAATTAAGGGAAACATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91