Detailed information of TA system TA241138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 698469..699115
• Replicon: chromosome
• Accession: NZ_CP095023
• Organism: Mycobacterium tuberculosis strain SGF0702019

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: MUW68_RS03130
• Protein ID: WP_003403137.1
• Coordinates: 698469..698882 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: MUW68_RS03135
• Protein ID: WP_003403139.1
• Coordinates: 698879..699115 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW68_RS03110 (MUW68_03110)	694552..696102	+	1551	WP_003905349.1	MCE family protein	-
MUW68_RS03115 (MUW68_03115)	696154..696546	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
MUW68_RS03120 (MUW68_03120)	696543..696800	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
MUW68_RS03125 (MUW68_03125)	696983..698218	-	1236	WP_003403128.1	ATP-binding protein	-
MUW68_RS03130 (MUW68_03130)	698469..698882	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUW68_RS03135 (MUW68_03135)	698879..699115	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
MUW68_RS03140 (MUW68_03140)	699219..699725	-	507	WP_003900973.1	two component system sensor kinase HK1	-
MUW68_RS03145 (MUW68_03145)	699839..700369	-	531	WP_003917320.1	two component system sensor kinase HK2	-
MUW68_RS03150 (MUW68_03150)	700353..701033	-	681	WP_244733088.1	two-component system response regulator TcrA	-
MUW68_RS03155 (MUW68_03155)	701156..701467	+	312	WP_003403164.1	hypothetical protein	-
MUW68_RS03160 (MUW68_03160)	701539..702489	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
MUW68_RS03165 (MUW68_03165)	702730..703314	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
MUW68_RS03170 (MUW68_03170)	703316..704026	+	711	Protein_629	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T241138 WP_003403137.1 NZ_CP095023:c698882-698469 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T241138 NZ_LT907988:c569079-568813 [Orrella dioscoreae]
ATGACTCCACCGCCCAAGGGCGTCTGTTTCGACAAGCATGGCTGGGAAGACTACCTCTCCTGGCAAGACGACCCGAAGAA
AAGAAAACGCATCAACCAGCTCATCGACCACACCTTGCGCACGCCTTTCGAAGGCATCGGCAAGCCCGAGCCCTTGAAAG
GCGACCTGCAGGGCTTCTGGTCACGGCGCATCGACGAGGAGCACCGGCTGGTCTACTGCGTGGAAGACGAACAACTGATC
GTCATCCAATGCCGATATCACTACTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT241138 WP_003403139.1 NZ_CP095023:c699115-698879 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT241138 NZ_LT907988:c569342-569076 [Orrella dioscoreae]
ATGAAAATCGTCAACTACACCGATGCCCGCAACGGCCTGAAAGCCGTCCTGGATCAGGTCAGCGATGACGCGGACGTCAC
CGTCATCACGCGCAGGGACGGGCCCGATGCCGTCGTCATGTCGAAGGCGCACTACGACGGCATCATGGAAACGCTCCACC
TCCTGCGCTCCCCGGCAAACGCGGCACGGCTGCGCGTGTCGGTGGCGAACGTCCGCGACAAGGCTTACCAGGCACGGAAT
CTCATCCATCCTGACGAGCAAGCATGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89