Detailed information of TA system TA241124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3172893..3173580
• Replicon: chromosome
• Accession: NZ_CP095022
• Organism: Mycobacterium tuberculosis strain SGF0472017

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: MUW67_RS15160
• Protein ID: WP_003414624.1
• Coordinates: 3173137..3173580 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: MUW67_RS15155
• Protein ID: WP_003414620.1
• Coordinates: 3172893..3173150 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW67_RS15135 (MUW67_15135)	3168213..3169067	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
MUW67_RS15140 (MUW67_15140)	3169123..3170286	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
MUW67_RS15145 (MUW67_15145)	3170303..3171517	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
MUW67_RS15150 (MUW67_15150)	3171525..3172766	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
MUW67_RS15155 (MUW67_15155)	3172893..3173150	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
MUW67_RS15160 (MUW67_15160)	3173137..3173580	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUW67_RS15165 (MUW67_15165)	3173660..3174322	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
MUW67_RS15170 (MUW67_15170)	3174418..3174609	+	192	WP_003414632.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
MUW67_RS15175 (MUW67_15175)	3174941..3176689	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
MUW67_RS15180 (MUW67_15180)	3176785..3177366	+	582	WP_003414644.1	fasciclin domain-containing protein	-
MUW67_RS15185 (MUW67_15185)	3177466..3177732	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T241124 WP_003414624.1 NZ_CP095022:3173137-3173580 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T241124 NZ_LT906615:369423-369710 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT241124 WP_003414620.1 NZ_CP095022:3172893-3173150 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT241124 NZ_LT906615:369721-370038 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• PDB: 7VWO
• AlphaFold DB: G0TFH0