Detailed information of TA system TA241122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3126321..3126925
• Replicon: chromosome
• Accession: NZ_CP095022
• Organism: Mycobacterium tuberculosis strain SGF0472017

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: MUW67_RS14935
• Protein ID: WP_003414492.1
• Coordinates: 3126321..3126713 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: MUW67_RS14940
• Protein ID: WP_003414495.1
• Coordinates: 3126710..3126925 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW67_RS14905 (MUW67_14905)	3121471..3122259	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
MUW67_RS14910 (MUW67_14910)	3122593..3123138	-	546	WP_014584866.1	DUF1802 family protein	-
MUW67_RS14915 (MUW67_14915)	3123410..3124294	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
MUW67_RS14920 (MUW67_14920)	3124297..3125184	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
MUW67_RS14925 (MUW67_14925)	3125489..3126034	-	546	WP_003910939.1	DUF1802 family protein	-
MUW67_RS14930 (MUW67_14930)	3126031..3126300	-	270	WP_003414489.1	DUF2277 family protein	-
MUW67_RS14935 (MUW67_14935)	3126321..3126713	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUW67_RS14940 (MUW67_14940)	3126710..3126925	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MUW67_RS14945 (MUW67_14945)	3126972..3127721	+	750	WP_003902358.1	enoyl-CoA hydratase	-
MUW67_RS14950 (MUW67_14950)	3127800..3128882	-	1083	WP_003414499.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
MUW67_RS14955 (MUW67_14955)	3128875..3130185	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
MUW67_RS14960 (MUW67_14960)	3130188..3131015	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
MUW67_RS14965 (MUW67_14965)	3131012..3131923	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T241122 WP_003414492.1 NZ_CP095022:c3126713-3126321 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T241122 NZ_LT906615:c349925-349626 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGAAACCATTTAATCTTACCGTCGCCGCCAAAGCCGATTTACGTGATATTGCTTTATTCACTCAACGACGCTGGGGAAA
AGAGCAGCGAAATGTTTATTTAAAGCAATTCGATGATTCCTTTTGGCTTTTAGCGGAAAATCCCGACATTGGTAAATCAT
GCGATGAAATCCGAGAGGGATACAGAAAATTTCCCCAAGGGAGTCACGTCATCTTTTATCAGCAAACCGGCAGCCAACAA
ATCAGGGTGATCCGAATTCTTCATAAGAGCATGGATGTGAACCCAATATTCGGCGCATAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT241122 WP_003414495.1 NZ_CP095022:c3126925-3126710 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT241122 NZ_LT906615:c350164-349922 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGGCTAAAAATACAAGTATCACTCTTGGTGAACACTTCGATGGCTTTATTACAAGCCAAATACAAAGTGGGCGTTACGG
CTCAGCAAGTGAAGTCATTCGCTCTGCGCTACGTCTACTCGAAAACCAAGAAACCAAACTACAGTCACTCCGTCAACTAC
TTATTGAAGGAGAGCAAAGTGGTGACGCTGATTATGACCTTGATAGCTTCATCAATGAACTCGATAGTGAAAACATTCGA
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8