Detailed information of TA system TA241120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3106123..3106693
• Replicon: chromosome
• Accession: NZ_CP095022
• Organism: Mycobacterium tuberculosis strain SGF0472017

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: MUW67_RS14810
• Protein ID: WP_003414166.1
• Coordinates: 3106123..3106479 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: MUW67_RS14815
• Protein ID: WP_003901465.1
• Coordinates: 3106463..3106693 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW67_RS14790 (MUW67_14790)	3101575..3103263	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
MUW67_RS14795 (MUW67_14795)	3103267..3103593	-	327	WP_003414157.1	hypothetical protein	-
MUW67_RS14800 (MUW67_14800)	3103766..3104353	+	588	WP_003914429.1	DUF3558 family protein	-
MUW67_RS14805 (MUW67_14805)	3104372..3106021	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
MUW67_RS14810 (MUW67_14810)	3106123..3106479	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
MUW67_RS14815 (MUW67_14815)	3106463..3106693	-	231	WP_003901465.1	type II toxin-antitoxin system antitoxin MazE9	Antitoxin
MUW67_RS14820 (MUW67_14820)	3106736..3107779	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
MUW67_RS14825 (MUW67_14825)	3107778..3108245	+	468	WP_003414177.1	DUF1778 domain-containing protein	-
MUW67_RS14830 (MUW67_14830)	3108421..3108675	-	255	WP_003917684.1	hypothetical protein	-
MUW67_RS14835 (MUW67_14835)	3108823..3109227	+	405	WP_003414181.1	hypothetical protein	-
MUW67_RS14840 (MUW67_14840)	3109224..3109415	+	192	WP_003414184.1	hypothetical protein	-
MUW67_RS14845 (MUW67_14845)	3109632..3109892	+	261	Protein_2928	transposase	-
MUW67_RS14850 (MUW67_14850)	3111002..3111259	+	258	WP_003899489.1	hypothetical protein	-
MUW67_RS14855 (MUW67_14855)	3111364..3111675	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T241120 WP_003414166.1 NZ_CP095022:c3106479-3106123 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T241120 NZ_LT906615:333949-334215 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT241120 WP_003901465.1 NZ_CP095022:c3106693-3106463 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT241120 NZ_LT906615:334202-334447 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3