Detailed information of TA system TA024112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 416496..417333
• Replicon: chromosome
• Accession: NC_012967
• Organism: Escherichia coli B str. REL606

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A140NB16
• Locus tag: ECB_RS01975
• Protein ID: WP_000227786.1
• Coordinates: 416496..417038 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: ECB_RS01980
• Protein ID: WP_001297137.1
• Coordinates: 417022..417333 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECB_RS01955	412026..412937	-	912	WP_000705853.1	2-dehydropantoate 2-reductase	-
ECB_RS01960	413105..413596	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
ECB_RS01965	413724..415088	-	1365	WP_001000978.1	MFS transporter	-
ECB_RS01970	415505..416440	+	936	WP_001297127.1	sel1 repeat family protein	-
ECB_RS01975	416496..417038	-	543	WP_000227786.1	GNAT family N-acetyltransferase	Toxin
ECB_RS01980	417022..417333	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
ECB_RS01985	417518..418408	-	891	WP_000971336.1	heme o synthase	-
ECB_RS01990	418420..418749	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
ECB_RS01995	418749..419363	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
ECB_RS02000	419353..421344	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
ECB_RS02005	421366..422313	-	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19794.98 Da
• Isoelectric Point: 8.3395

>T24112 WP_000227786.1 NC_012967:c417038-416496 [Escherichia coli B str. REL606]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T24112 NC_012967:c417038-416496 [Escherichia coli B str. REL606]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACTCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT24112 WP_001297137.1 NC_012967:c417333-417022 [Escherichia coli B str. REL606]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT24112 NC_012967:c417333-417022 [Escherichia coli B str. REL606]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A140NB16

Antitoxin

• AlphaFold DB: A0A0E0Y6B3