Detailed information of TA system TA241116

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2927135..2927849
• Replicon: chromosome
• Accession: NZ_CP095022
• Organism: Mycobacterium tuberculosis strain SGF0472017

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQK0
• Locus tag: MUW67_RS13755
• Protein ID: WP_003413460.1
• Coordinates: 2927409..2927849 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ20
• Locus tag: MUW67_RS13750
• Protein ID: WP_003413456.1
• Coordinates: 2927135..2927422 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW67_RS13715 (MUW67_13715)	2922557..2922802	+	246	WP_003413429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
MUW67_RS13720 (MUW67_13720)	2922799..2923203	+	405	WP_003413432.1	type II toxin-antitoxin system VapC family toxin	-
MUW67_RS13725 (MUW67_13725)	2923420..2924040	+	621	WP_003413441.1	DUF4178 domain-containing protein	-
MUW67_RS13730 (MUW67_13730)	2924051..2924545	+	495	WP_003413444.1	DUF2617 family protein	-
MUW67_RS13735 (MUW67_13735)	2924542..2924973	+	432	WP_003899390.1	DUF4247 domain-containing protein	-
MUW67_RS13740 (MUW67_13740)	2924998..2925456	+	459	WP_003413451.1	DUF350 domain-containing protein	-
MUW67_RS13745 (MUW67_13745)	2925453..2927024	+	1572	WP_003899392.1	polyamine aminopropyltransferase	-
MUW67_RS13750 (MUW67_13750)	2927135..2927422	+	288	WP_003413456.1	antitoxin VapB41	Antitoxin
MUW67_RS13755 (MUW67_13755)	2927409..2927849	+	441	WP_003413460.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUW67_RS13760 (MUW67_13760)	2927870..2928625	-	756	WP_003413464.1	YebC/PmpR family DNA-binding transcriptional regulator	-
MUW67_RS13765 (MUW67_13765)	2928758..2929354	-	597	WP_003413465.1	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT	-
MUW67_RS13770 (MUW67_13770)	2929362..2930207	-	846	WP_003413466.1	acyl-CoA thioesterase II	-
MUW67_RS13775 (MUW67_13775)	2930236..2931135	-	900	WP_003413468.1	pyridoxal 5'-phosphate synthase lyase subunit PdxS	-
MUW67_RS13780 (MUW67_13780)	2931263..2931937	+	675	WP_003413471.1	pyridoxamine 5'-phosphate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-146)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16026.38 Da
• Isoelectric Point: 6.8599

>T241116 WP_003413460.1 NZ_CP095022:2927409-2927849 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Nucleotide

• Length: 441 bp

>T241116 NZ_LT906556:c127651-127493 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10453.98 Da
• Isoelectric Point: 7.2797

>AT241116 WP_003413456.1 NZ_CP095022:2927135-2927422 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Nucleotide

• Length: 288 bp

>AT241116 NZ_LT906556:c127919-127695 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: G0TQK0

Antitoxin

• AlphaFold DB: A0A7U4BWB8