Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2249229..2249855 Replicon chromosome
Accession NZ_CP095022
Organism Mycobacterium tuberculosis strain SGF0472017

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag MUW67_RS10565 Protein ID WP_003410075.1
Coordinates 2249457..2249855 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A806JLL8
Locus tag MUW67_RS10560 Protein ID WP_003911750.1
Coordinates 2249229..2249456 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW67_RS10550 (MUW67_10550) 2247268..2247612 - 345 WP_003410065.1 ferredoxin family protein -
MUW67_RS10555 (MUW67_10555) 2247801..2248970 - 1170 WP_003899126.1 ATP-binding protein -
MUW67_RS10560 (MUW67_10560) 2249229..2249456 + 228 WP_003911750.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MUW67_RS10565 (MUW67_10565) 2249457..2249855 + 399 WP_003410075.1 PIN domain nuclease Toxin
MUW67_RS10570 (MUW67_10570) 2250038..2250469 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
MUW67_RS10575 (MUW67_10575) 2250570..2251004 + 435 WP_003914358.1 DUF1398 domain-containing protein -
MUW67_RS10580 (MUW67_10580) 2251441..2251620 - 180 Protein_2089 hypothetical protein -
MUW67_RS10585 (MUW67_10585) 2251708..2252328 + 621 WP_003410086.1 IS110 family transposase -
MUW67_RS10590 (MUW67_10590) 2252282..2252872 + 591 WP_003899131.1 IS110 family transposase -
MUW67_RS10595 (MUW67_10595) 2253000..2254076 - 1077 WP_280518135.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T241106 WP_003410075.1 NZ_CP095022:2249457-2249855 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T241106 NZ_LT906479:4259367-4259753 [Serratia ficaria]
ATGGACATTTTCGTTACGGATGATTTCGACAAATTTATGAAGAAAAATCGCATCGTCGATCAAAAAATTTGCACGGCTGC
TCACGAACTCGAACATGGCAACCATGACGGCGATCTGGGGGGCGGCGTCTACAAGAAACGCCTCCCGCTCAATCGGGGTA
AACGGGGCGGCGCCCGATCCATCGTGGCATTTAAACACGGACGCCATCAGTATTTTGTTGATGGATGGCTAAAAAACACC
GTAAAACAGAACGGTGCAAAAGAAATTAACGATGATGAATTAGCAACCTATCGCGAGCTCGCGAAAGACTTTCTTGCCAT
GCCGCCAGAAATTATCAAACGAGCCATCGATAGCGGTTACCTGCGAGAGGTGAAATGCGATGACTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8346.16 Da        Isoelectric Point: 4.1621

>AT241106 WP_003911750.1 NZ_CP095022:2249229-2249456 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 228 bp

>AT241106 NZ_LT906479:4259746-4260090 [Serratia ficaria]
ATGACTGATAATCGCCTACTCAGACTGCAAAAAATGGCGCAACGATTCAATGAAATTGGCGCAGTGTCAGACGACACCAT
GCGCAGCATCGATGCGCGCGTTCAGGCGCAAGAGCAAAATGCCCAGCATGAACAGCGTGAGCAAATGGACGGCGCACGCA
TCAAAATGCTACGCAAACGGCTTGGCTTAAGTCAGGCCGATCTGGCGGCCGTGGTCAATATGTCGACCACCAGCGTACAG
AAATGGGAACGTAATGCGGTGAAACCCCAGGGTTCCGCGCTCAGAATGCTGGAAATTATCGAGCAGAAAGGCGTCGCCAG
CCTGATTATTCCGCCACGAAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
AlphaFold DB A0A806JLL8

References