Detailed information of TA system TA241100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2181334..2182037
• Replicon: chromosome
• Accession: NZ_CP095022
• Organism: Mycobacterium tuberculosis strain SGF0472017

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: MUW67_RS10150
• Protein ID: WP_003409778.1
• Coordinates: 2181334..2181663 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: MUW67_RS10155
• Protein ID: WP_003409780.1
• Coordinates: 2181660..2182037 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW67_RS10130 (MUW67_10130)	2177717..2178787	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
MUW67_RS10135 (MUW67_10135)	2178784..2179299	+	516	WP_003409718.1	flavin reductase family protein	-
MUW67_RS10140 (MUW67_10140)	2179296..2180357	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
MUW67_RS10145 (MUW67_10145)	2180354..2181124	+	771	WP_003409775.1	SDR family oxidoreductase	-
MUW67_RS10150 (MUW67_10150)	2181334..2181663	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUW67_RS10155 (MUW67_10155)	2181660..2182037	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
MUW67_RS10160 (MUW67_10160)	2182034..2182624	-	591	WP_003409784.1	SEC-C metal-binding domain-containing protein	-
MUW67_RS10165 (MUW67_10165)	2182679..2184042	+	1364	Protein_2005	HNH endonuclease signature motif containing protein	-
MUW67_RS10170 (MUW67_10170)	2184197..2184649	-	453	WP_003899095.1	lipoprotein	-
MUW67_RS10175 (MUW67_10175)	2184713..2185114	+	402	WP_003409869.1	hypothetical protein	-
MUW67_RS10180 (MUW67_10180)	2185107..2185289	-	183	WP_003409870.1	hypothetical protein	-
MUW67_RS10185 (MUW67_10185)	2185403..2185753	-	351	WP_003409871.1	hypothetical protein	-
MUW67_RS10190 (MUW67_10190)	2185764..2186666	-	903	WP_003899097.1	hypothetical protein	-
MUW67_RS10195 (MUW67_10195)	2186687..2186878	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T241100 WP_003409778.1 NZ_CP095022:c2181663-2181334 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T241100 NZ_LT906479:592587-592862 [Serratia ficaria]
ATGCGTATCGAATGGGATAATGCAGCGCTACGGGACAGGGAACGTATTTTTGAGTTTCTTTATATTTTCAATCCACAGGC
GGCAGAGCAGGCTGATAACGAGATCGATAAAGCGGTAAAGCGCCTGCTGGATCATCCTGAGTTAGGTAAGCCCTGGTATG
GGAAGGCACGTAAGCTGCTAATTAACAAGGCCTCCCTGTTGCTGGTGTATATCATCGTTGATGACGTTATTAAGGTTTTC
TCGATTGCCCACCAGCGTGAGAAATTTCCAAACTAG

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT241100 WP_003409780.1 NZ_CP095022:c2182037-2181660 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT241100 NZ_LT906479:592291-592578 [Serratia ficaria]
ATGGAAAGTCGTATCCAGTTTCGCATCGAAGATGAAACCAAGCGTTTGGCGCAGAAGGCCGCTGAGGCTAAAGGAACGAC
ATTGAGTGAAGCCTGCCGCCGCTTAGCGGAGCAAATGGCTGATGAGCAACGGGCCGCGGAGGAGCACGAAAACTGGCTGA
AAGATAAGGTGGATGCAGCTTTTTCCCGTCTGCATGAAGGTAATGCTGTCTACCTTAGCCAACAAGAAGTAGAAGAAAGC
ATGGATGCCTTTAAGGCAAAAATTAGAGCGAAATACGACCGAAAATAG

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8