Detailed information of TA system TA241091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1238068..1238636
• Replicon: chromosome
• Accession: NZ_CP095022
• Organism: Mycobacterium tuberculosis strain SGF0472017

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: MUW67_RS05945
• Protein ID: WP_003405865.1
• Coordinates: 1238262..1238636 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: MUW67_RS05940
• Protein ID: WP_003405863.1
• Coordinates: 1238068..1238265 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW67_RS05920 (MUW67_05920)	1234109..1234747	-	639	WP_003898730.1	lipid droplet-associated protein	-
MUW67_RS05925 (MUW67_05925)	1234837..1235844	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
MUW67_RS05930 (MUW67_05930)	1235861..1236844	-	984	WP_003898731.1	hypothetical protein	-
MUW67_RS05935 (MUW67_05935)	1236907..1237980	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
MUW67_RS05940 (MUW67_05940)	1238068..1238265	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MUW67_RS05945 (MUW67_05945)	1238262..1238636	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUW67_RS05950 (MUW67_05950)	1238839..1239537	+	699	WP_003898733.1	hypothetical protein	-
MUW67_RS05955 (MUW67_05955)	1239655..1239840	+	186	WP_003901093.1	hypothetical protein	-
MUW67_RS05960 (MUW67_05960)	1239767..1240042	-	276	WP_003405867.1	hypothetical protein	-
MUW67_RS05965 (MUW67_05965)	1240285..1240608	+	324	WP_003405871.1	putative quinol monooxygenase	-
MUW67_RS05970 (MUW67_05970)	1240623..1241321	-	699	WP_031646953.1	hypothetical protein	-
MUW67_RS05975 (MUW67_05975)	1241355..1241564	+	210	WP_003911400.1	hypothetical protein	-
MUW67_RS05980 (MUW67_05980)	1241516..1242286	-	771	Protein_1176	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T241091 WP_003405865.1 NZ_CP095022:1238262-1238636 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T241091 NZ_LT906474:3643295-3643636 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT241091 WP_003405863.1 NZ_CP095022:1238068-1238265 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT241091 NZ_LT906474:3642957-3643274 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07