Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapC-mazE/PIN(toxin)
Location 753867..754659 Replicon chromosome
Accession NZ_CP095022
Organism Mycobacterium tuberculosis strain SGF0472017

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB2
Locus tag MUW67_RS03490 Protein ID WP_003403386.1
Coordinates 754222..754659 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag MUW67_RS03485 Protein ID WP_003403381.1
Coordinates 753867..754112 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW67_RS03455 (MUW67_03455) 748887..750392 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
MUW67_RS03460 (MUW67_03460) 750473..751483 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
MUW67_RS03465 (MUW67_03465) 751871..752254 - 384 WP_003403365.1 ribonuclease VapC6 -
MUW67_RS03470 (MUW67_03470) 752349..752504 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
MUW67_RS03475 (MUW67_03475) 752580..753296 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
MUW67_RS03480 (MUW67_03480) 753572..753880 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
MUW67_RS03485 (MUW67_03485) 753867..754112 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
MUW67_RS03490 (MUW67_03490) 754222..754659 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin Toxin
MUW67_RS03495 (MUW67_03495) 754656..754910 - 255 WP_003911263.1 antitoxin VapB7 -
MUW67_RS03500 (MUW67_03500) 755024..757387 + 2364 WP_003901895.1 arylsulfatase AtsD -
MUW67_RS03505 (MUW67_03505) 757450..757776 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MUW67_RS03510 (MUW67_03510) 757688..758026 + 339 WP_003403405.1 PIN domain-containing protein -
MUW67_RS03515 (MUW67_03515) 758023..758196 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T241085 WP_003403386.1 NZ_CP095022:c754659-754222 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T241085 NZ_LT906474:c2427949-2427773 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT241085 WP_003403381.1 NZ_CP095022:c754112-753867 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT241085 NZ_LT906474:c2427727-2427311 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSW5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References