Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 714764..715413 Replicon chromosome
Accession NZ_CP095022
Organism Mycobacterium tuberculosis strain SGF0472017

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag MUW67_RS03280 Protein ID WP_003403236.1
Coordinates 715018..715413 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag MUW67_RS03275 Protein ID WP_003403235.1
Coordinates 714764..715018 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW67_RS03250 (MUW67_03250) 709890..709973 + 84 Protein_643 galactose-1-phosphate uridylyltransferase -
MUW67_RS03255 (MUW67_03255) 709992..711073 + 1082 Protein_644 galactose-1-phosphate uridylyltransferase -
MUW67_RS03260 (MUW67_03260) 711070..712161 + 1092 WP_003900977.1 galactokinase -
MUW67_RS03265 (MUW67_03265) 712556..713620 + 1065 WP_003898532.1 zinc ribbon domain-containing protein -
MUW67_RS03270 (MUW67_03270) 713724..714671 + 948 WP_003403232.1 DUF732 domain-containing protein -
MUW67_RS03275 (MUW67_03275) 714764..715018 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
MUW67_RS03280 (MUW67_03280) 715018..715413 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
MUW67_RS03285 (MUW67_03285) 715507..716247 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
MUW67_RS03290 (MUW67_03290) 716379..716639 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MUW67_RS03295 (MUW67_03295) 716636..717043 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
MUW67_RS03300 (MUW67_03300) 717115..718266 - 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
MUW67_RS03305 (MUW67_03305) 718359..720086 - 1728 WP_003901883.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T241081 WP_003403236.1 NZ_CP095022:715018-715413 [Mycobacterium tuberculosis]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T241081 NZ_LT906474:1812684-1812938 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT241081 WP_003403235.1 NZ_CP095022:714764-715018 [Mycobacterium tuberculosis]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT241081 NZ_LT906474:1812436-1812687 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References