Detailed information of TA system TA241078

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 695508..696154
• Replicon: chromosome
• Accession: NZ_CP095022
• Organism: Mycobacterium tuberculosis strain SGF0472017

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: MUW67_RS03140
• Protein ID: WP_003403137.1
• Coordinates: 695508..695921 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: MUW67_RS03145
• Protein ID: WP_003403139.1
• Coordinates: 695918..696154 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW67_RS03120 (MUW67_03120)	691591..693141	+	1551	WP_003905349.1	MCE family protein	-
MUW67_RS03125 (MUW67_03125)	693193..693585	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
MUW67_RS03130 (MUW67_03130)	693582..693839	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
MUW67_RS03135 (MUW67_03135)	694022..695257	-	1236	WP_003403128.1	ATP-binding protein	-
MUW67_RS03140 (MUW67_03140)	695508..695921	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUW67_RS03145 (MUW67_03145)	695918..696154	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
MUW67_RS03150 (MUW67_03150)	696258..696764	-	507	WP_003900973.1	two component system sensor kinase HK1	-
MUW67_RS03155 (MUW67_03155)	696878..697408	-	531	WP_003917320.1	two component system sensor kinase HK2	-
MUW67_RS03160 (MUW67_03160)	697392..698087	-	696	WP_003918099.1	two-component system response regulator TcrA	-
MUW67_RS03165 (MUW67_03165)	698210..698521	+	312	WP_003403164.1	hypothetical protein	-
MUW67_RS03170 (MUW67_03170)	698593..699543	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
MUW67_RS03175 (MUW67_03175)	699784..700368	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
MUW67_RS03180 (MUW67_03180)	700370..701080	+	711	Protein_629	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T241078 WP_003403137.1 NZ_CP095022:c695921-695508 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T241078 NZ_LT906474:883319-883726 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT241078 WP_003403139.1 NZ_CP095022:c696154-695918 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT241078 NZ_LT906474:883072-883338 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89