Detailed information of TA system TA241075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 638587..639263
• Replicon: chromosome
• Accession: NZ_CP095022
• Organism: Mycobacterium tuberculosis strain SGF0472017

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: MUW67_RS02890
• Protein ID: WP_003898500.1
• Coordinates: 638587..639000 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: MUW67_RS02895
• Protein ID: WP_003402915.1
• Coordinates: 638997..639263 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW67_RS02860 (MUW67_02860)	633974..634276	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
MUW67_RS02865 (MUW67_02865)	634336..634614	-	279	WP_003402901.1	hypothetical protein	-
MUW67_RS02870 (MUW67_02870)	634611..635864	-	1254	WP_003402904.1	inorganic phosphate transporter	-
MUW67_RS02875 (MUW67_02875)	635984..636370	-	387	WP_003402907.1	VOC family protein	-
MUW67_RS02880 (MUW67_02880)	636433..637275	-	843	WP_031724453.1	SDR family oxidoreductase	-
MUW67_RS02885 (MUW67_02885)	637371..638273	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
MUW67_RS02890 (MUW67_02890)	638587..639000	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUW67_RS02895 (MUW67_02895)	638997..639263	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
MUW67_RS02900 (MUW67_02900)	639455..641170	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
MUW67_RS02905 (MUW67_02905)	641248..642852	+	1605	WP_003402920.1	amidohydrolase	-
MUW67_RS02910 (MUW67_02910)	642849..643829	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T241075 WP_003898500.1 NZ_CP095022:c639000-638587 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T241075 NZ_LT906474:c641364-640900 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT241075 WP_003402915.1 NZ_CP095022:c639263-638997 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT241075 NZ_LT906474:c641699-641364 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0