Detailed information of TA system TA241064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3166333..3167020
• Replicon: chromosome
• Accession: NZ_CP095021
• Organism: Mycobacterium tuberculosis strain SGF0422017

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: MUW66_RS15120
• Protein ID: WP_003414624.1
• Coordinates: 3166577..3167020 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: MUW66_RS15115
• Protein ID: WP_003414620.1
• Coordinates: 3166333..3166590 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW66_RS15095 (MUW66_15095)	3161653..3162507	-	855	WP_244743680.1	GNAT family N-acetyltransferase	-
MUW66_RS15100 (MUW66_15100)	3162563..3163726	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
MUW66_RS15105 (MUW66_15105)	3163743..3164957	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
MUW66_RS15110 (MUW66_15110)	3164965..3166206	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
MUW66_RS15115 (MUW66_15115)	3166333..3166590	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
MUW66_RS15120 (MUW66_15120)	3166577..3167020	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUW66_RS15125 (MUW66_15125)	3167100..3167762	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
MUW66_RS15130 (MUW66_15130)	3167858..3168049	+	192	WP_003414632.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
MUW66_RS15135 (MUW66_15135)	3168381..3170129	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
MUW66_RS15140 (MUW66_15140)	3170225..3170806	+	582	WP_003414644.1	fasciclin domain-containing protein	-
MUW66_RS15145 (MUW66_15145)	3170906..3171172	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T241064 WP_003414624.1 NZ_CP095021:3166577-3167020 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T241064 NZ_LT906469:c1569571-1569188 [Mycolicibacter terrae]
TTGACCGAATTTCTCGACCGCGATGACCTGCTCGTCGCCGGGGCGTTCGCCGTGAGCCACGCCTGCGAAGTCGGGGACTA
CGGGTTGCTCGACGCCGCAGTCGCCCGGCCTCAAGCCACGGTCTTCGGTGTGGACGCCTATCCCGATCTGTACGCGAAGG
CGGCGGCGTTGCTGCAATCACTGGCCCGCAACCATGCCCTGGTGGACGGCAACATGCGCACCGCCTGGGCGTCGGCATGG
ACGTTTCTGTACCTCAACGCCTGTGAGCTCTCCCCGGACTTCGACGTCGATGCGGCCGAGGTGTTCATGAACGACGTGGC
ACAGCGCGGAGAGCTGCCGGTCGAGGACATCGCCGCCACGCTGGCATCATTCGCCGTTACGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT241064 WP_003414620.1 NZ_CP095021:3166333-3166590 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT241064 NZ_LT906469:c1569765-1569568 [Mycolicibacter terrae]
GTGCCGTCGCTGAACATCACCTTCACCGAGGAGGAACTGGCCGCCGTCCGCGCCGCGGCCGGGGAACAGAACCTCTCGCT
GCGGGTATTCGCGCACCGGGCGGTCGTCACCGCCGCCAGCGACCACCGGCGCCGCGTCGCCGAAGCGGCAGCGCTGGTCG
CACAACGCTCCGCCGAGTTGAATCGCCGCCTGGCTTGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• PDB: 7VWO
• AlphaFold DB: G0TFH0