Detailed information of TA system TA241062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3119815..3120419
• Replicon: chromosome
• Accession: NZ_CP095021
• Organism: Mycobacterium tuberculosis strain SGF0422017

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: MUW66_RS14895
• Protein ID: WP_003414492.1
• Coordinates: 3119815..3120207 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: MUW66_RS14900
• Protein ID: WP_003414495.1
• Coordinates: 3120204..3120419 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW66_RS14865 (MUW66_14865)	3114965..3115753	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
MUW66_RS14870 (MUW66_14870)	3116087..3116632	-	546	WP_014584866.1	DUF1802 family protein	-
MUW66_RS14875 (MUW66_14875)	3116904..3117788	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
MUW66_RS14880 (MUW66_14880)	3117791..3118678	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
MUW66_RS14885 (MUW66_14885)	3118983..3119528	-	546	WP_003910939.1	DUF1802 family protein	-
MUW66_RS14890 (MUW66_14890)	3119525..3119794	-	270	WP_003414489.1	DUF2277 family protein	-
MUW66_RS14895 (MUW66_14895)	3119815..3120207	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUW66_RS14900 (MUW66_14900)	3120204..3120419	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MUW66_RS14905 (MUW66_14905)	3120466..3121215	+	750	WP_003902358.1	enoyl-CoA hydratase	-
MUW66_RS14910 (MUW66_14910)	3121294..3122376	-	1083	WP_003414499.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
MUW66_RS14915 (MUW66_14915)	3122369..3123679	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
MUW66_RS14920 (MUW66_14920)	3123682..3124509	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
MUW66_RS14925 (MUW66_14925)	3124506..3125417	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T241062 WP_003414492.1 NZ_CP095021:c3120207-3119815 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T241062 NZ_LT906464:c1599397-1599176 [Staphylococcus muscae]
GTGCATAGTAAAGTATGGCTATCTGGTCTCTCGAGTGAGGAACAGAAGAAACAGCTTGAGTCGAGACTGAGCAATATGAT
TGGTGTTGAAGCGGTAGAAGTGAATACCGAATCACAAATGGTGACACTGACGTATGAAACACCAGCTAACTTGAATACGT
TGGAAAAAGAAATTTATGATGCAGGTTATCCTGTCATCAATTCATATAAAGGAGAGCGTTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT241062 WP_003414495.1 NZ_CP095021:c3120419-3120204 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT241062 NZ_LT906464:c1599698-1599411 [Staphylococcus muscae]
ATGACAGAACATGCAAATCATTCTGACGTGACGAAACGCAATTTACAATCTCGTTTAAATCGCATTGAAGGACAAGTACG
AGCAATTAATCGTATGATTGAAGAAGACGTCTATTGCGATGATGTTCTGACTCAAATCCGTGCAACACGTTCTGCGCTGA
ATGGTGTGGCATCCAAGTTGTTAGAACACCATATGAAAGGATGTATCATGCACAAAGTAGAAGGTGGCAATGAACATGAA
GCGATGGATGAACTACTCGTGACCTTCCAAAAGTTGATGAAAGACTAG

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8