Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3059634..3060313 Replicon chromosome
Accession NZ_CP095021
Organism Mycobacterium tuberculosis strain SGF0422017

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag MUW66_RS14540 Protein ID WP_003414059.1
Coordinates 3059634..3060050 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag MUW66_RS14545 Protein ID WP_003414061.1
Coordinates 3060047..3060313 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW66_RS14515 (MUW66_14515) 3055686..3056588 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
MUW66_RS14520 (MUW66_14520) 3056657..3057409 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
MUW66_RS14530 (MUW66_14530) 3057653..3057928 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
MUW66_RS14535 (MUW66_14535) 3057925..3059547 - 1623 WP_003414057.1 class I SAM-dependent DNA methyltransferase -
MUW66_RS14540 (MUW66_14540) 3059634..3060050 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
MUW66_RS14545 (MUW66_14545) 3060047..3060313 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MUW66_RS14550 (MUW66_14550) 3060339..3060734 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
MUW66_RS14555 (MUW66_14555) 3060731..3061000 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
MUW66_RS14560 (MUW66_14560) 3061010..3062104 - 1095 WP_003414068.1 restriction endonuclease subunit S -
MUW66_RS14565 (MUW66_14565) 3062101..3062520 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
MUW66_RS14570 (MUW66_14570) 3062519..3062593 + 75 Protein_2876 hypothetical protein -
MUW66_RS14575 (MUW66_14575) 3062594..3063073 - 480 WP_003414073.1 dihydrofolate reductase -
MUW66_RS14580 (MUW66_14580) 3063144..3063944 - 801 WP_003911953.1 thymidylate synthase -
MUW66_RS14585 (MUW66_14585) 3064100..3064837 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-123)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T241058 WP_003414059.1 NZ_CP095021:c3060050-3059634 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T241058 NZ_LT906462:680913-681284 [Mammaliicoccus stepanovicii]
ATGAGAAGAGGAGATGTGTATCTCGCAGATCTTTCACCAGTTACTGGTTCTGAACAAGGGGGAACAAGACCAGTCGTGAT
TATACAAAATGATACCGGAAATAGATACAGTCCAACTGTCATTGTAGCAGCGATAACTGGGAAGATTAACAAAGCCAAAA
TACCTACACACGTAGAAATTGAAGCAGCGAAATACAAATTAGATAGAGATTCAGTTATTTTACTTGAACAGATAAGAACA
ATCGATAAAAAACGTTTAAAAGAAAAATTAACATATTTATCTGATGCAAAGATGAAAGAGGTTGATTTAGCAATTGCAAT
CAGCCTCAATCTCACTTTGCAACATAAGTTTGATACATTAGGTAATACATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT241058 WP_003414061.1 NZ_CP095021:c3060313-3060047 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT241058 NZ_LT906462:680745-680912 [Mammaliicoccus stepanovicii]
ATGACATCTTTAACACAAAATCGCACGCAAAATTTAGAACAAACGCTTAAAGAAGGATATCGTTCTATGGCAGATTTGAA
CCTCTCCCTTGCATCTGAAGCATACTATAGTGAATGTGAAGCATGTGATTCAAATGAGTCGCATTTGACATCTTCAAACG
GTGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5SV2


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References