Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2860244..2860881 Replicon chromosome
Accession NZ_CP095021
Organism Mycobacterium tuberculosis strain SGF0422017

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag MUW66_RS13450 Protein ID WP_003413180.1
Coordinates 2860244..2860639 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag MUW66_RS13455 Protein ID WP_003413183.1
Coordinates 2860636..2860881 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW66_RS13410 (MUW66_13410) 2855648..2856859 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
MUW66_RS13415 (MUW66_13415) 2856986..2857645 + 660 WP_003902296.1 LppA family lipoprotein -
MUW66_RS13420 (MUW66_13420) 2857642..2858304 + 663 WP_003905878.1 LppA family lipoprotein -
MUW66_RS13425 (MUW66_13425) 2858301..2858578 + 278 Protein_2652 type II toxin-antitoxin system VapB family antitoxin -
MUW66_RS13430 (MUW66_13430) 2858671..2859084 + 414 WP_003413164.1 PIN domain nuclease -
MUW66_RS13435 (MUW66_13435) 2859123..2859380 + 258 WP_003413167.1 CopG family transcriptional regulator -
MUW66_RS13440 (MUW66_13440) 2859377..2859754 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
MUW66_RS13445 (MUW66_13445) 2859770..2860144 - 375 WP_003413177.1 hypothetical protein -
MUW66_RS13450 (MUW66_13450) 2860244..2860639 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
MUW66_RS13455 (MUW66_13455) 2860636..2860881 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
MUW66_RS13460 (MUW66_13460) 2861292..2861711 - 420 WP_003413190.1 A24 family peptidase -
MUW66_RS13465 (MUW66_13465) 2861723..2862532 - 810 WP_003413193.1 shikimate dehydrogenase -
MUW66_RS13470 (MUW66_13470) 2862529..2863782 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
MUW66_RS13475 (MUW66_13475) 2863775..2864287 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T241055 WP_003413180.1 NZ_CP095021:c2860639-2860244 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T241055 NZ_LT906460:2505476-2505571 [Staphylococcus simiae]
ATGCTAGAAACCCTTGTCAACACTGCTACCACAGTTATCAGTGGTTGTATTATTGCGCTATTTACGCACTGGCTACGTAA
TCGCAATAATAAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT241055 WP_003413183.1 NZ_CP095021:c2860881-2860636 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT241055 NZ_LT906460:c2505634-2505596 [Staphylococcus simiae]
TATGCACCTAGTCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References