Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2319117..2319753 Replicon chromosome
Accession NZ_CP095021
Organism Mycobacterium tuberculosis strain SGF0422017

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag MUW66_RS10855 Protein ID WP_003410654.1
Coordinates 2319343..2319753 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag MUW66_RS10850 Protein ID WP_003410651.1
Coordinates 2319117..2319350 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW66_RS10835 (MUW66_10835) 2314574..2314966 + 393 WP_229303073.1 metal ABC transporter permease -
MUW66_RS10840 (MUW66_10840) 2314967..2315371 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
MUW66_RS10845 (MUW66_10845) 2315455..2319039 - 3585 WP_003910889.1 cobaltochelatase subunit CobN -
MUW66_RS10850 (MUW66_10850) 2319117..2319350 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
MUW66_RS10855 (MUW66_10855) 2319343..2319753 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
MUW66_RS10860 (MUW66_10860) 2319737..2320828 + 1092 WP_003900454.1 precorrin-3B synthase -
MUW66_RS10865 (MUW66_10865) 2320838..2321464 + 627 WP_003410658.1 precorrin-8X methylmutase -
MUW66_RS10870 (MUW66_10870) 2321461..2322987 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
MUW66_RS10875 (MUW66_10875) 2322933..2324156 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T241048 WP_003410654.1 NZ_CP095021:2319343-2319753 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T241048 NZ_LT906457:c632057-631200 [Legionella spiritensis]
ATGATGAAAGATATCAGTGCTTCTATCCGAGCACGACTAAAAAATATCGCGAAAGAAGAACAAACTGACTTCAATGCAGT
ATTAACCCGTTATGGTCTTGAGCGTTTGCTATACCGAATTGGTCAATCTGAATATGCCAATCAATATTTATTGAAAGGTG
CTTTACTATTTAACTTATGGTACGACATGCCACATCGACCTACTTTGGATATTGACTTGTTAGGATTTGGTAATAACGAA
CTTGATTATTTGACAGGTGTTTTCAAAGAAATTTGCATAATTTCTGCTGCTGATGGAATCATTTTTGATGAGAAATCTGT
TTCAGCAAATACTATAAAAAAAGAGGGTGGATATACAGGTGCCAGAATCGAGTTATTTGCTGAATTGTCAAAGGCACGTA
TTAAAATACAAATTGATATTGGTTATGGTGATGTTGTAACGCCCGGCCCAATTGATTTGAATTATCCTGTTTTGATAAGT
GATCTACCTCCGCCAAAAATTCGAACCTATCCAATTTATACAGTGATTGCTGAAAAGCTGCATGCAATTGCCTTATTGGG
TATGGCAAATAGTCGATTAAAAGATTATCTGGATCTATATGTATTATTGAAAAATGAAGAACTAGACGAAGAAACAGTGA
TAGATGCTATCATTTCTACCTTCACTCAAAGAGGGATGACTATTCCTAAAAATCTACCAATTGGTTTGACCTCAGAATAT
AGTCAGGATGGATCGAGACAAGCAATGTGGAATTCTTTCTTAAATAAAAATGAAGTTGAACTCAAACCGTTGCCAGAGGC
CGTGTCTTCGATAGGAGATTATATCCAGCCAATTCTTGTTAAAGCTTCTACAAGATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT241048 WP_003410651.1 NZ_CP095021:2319117-2319350 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT241048 NZ_LT906457:c632662-632054 [Legionella spiritensis]
ATGAATGCAGAGAAAAATTATAAACAAACAGTTCTCAATATGGTTAGAGAAAAGGGAATTATTAAAATTTCTGAGCTAAC
CAAAAAGGGAATAACCAGAGCGACAGTTTCAAGGCTTGTAAGCGAAAACAAACTTGAAAAAATTGCAACAGGACTCTATT
GTCTACCAGAAACTGAGTTCTCAGAAAAAGAAAGTCTAGTTATTCTAGCAAGCCGAGTACCGCAAGCAGTATTTTGTCTA
CTAACAGCCCTGCAAATTCATGAGCTAACAACGCAACTACCACGAAAGGTTTGGATTGCAATGCCTAGAGGTAGTCACAC
ACCAAAGATTGATTATCCCCCTGTGAAAATGATTCAGTACTCAAAAGAAGTTTATGAAGAAGGTATTGAAACAATAGAAT
CAGATAATATTAAATTGAGAGTTTACAATTCTGCCAAAACAATAGCTGATTGCTTTAAGCACCGTACAACAATTGGTATT
GATGTAGCTATTGAAGCACTAAAAGAAGCGTATTCAAAAAACAAAGTTTCAATGGATGAGCTATGGCATTATGCGAAAAT
ATGTCGTGTTGCCAATATAATGCGTCCTTATCTAGAAGCTATCCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References