Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2249974..2250600 Replicon chromosome
Accession NZ_CP095021
Organism Mycobacterium tuberculosis strain SGF0422017

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag MUW66_RS10555 Protein ID WP_003410075.1
Coordinates 2250202..2250600 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A806JLL8
Locus tag MUW66_RS10550 Protein ID WP_003911750.1
Coordinates 2249974..2250201 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW66_RS10540 (MUW66_10540) 2248013..2248357 - 345 WP_003410065.1 ferredoxin family protein -
MUW66_RS10545 (MUW66_10545) 2248546..2249715 - 1170 WP_003899126.1 ATP-binding protein -
MUW66_RS10550 (MUW66_10550) 2249974..2250201 + 228 WP_003911750.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MUW66_RS10555 (MUW66_10555) 2250202..2250600 + 399 WP_003410075.1 PIN domain nuclease Toxin
MUW66_RS10560 (MUW66_10560) 2250783..2251214 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
MUW66_RS10565 (MUW66_10565) 2251315..2251749 + 435 WP_003914358.1 DUF1398 domain-containing protein -
MUW66_RS10570 (MUW66_10570) 2252186..2252365 - 180 Protein_2089 hypothetical protein -
MUW66_RS10575 (MUW66_10575) 2252453..2253073 + 621 WP_003410086.1 IS110 family transposase -
MUW66_RS10580 (MUW66_10580) 2253027..2253617 + 591 WP_003899131.1 IS110 family transposase -
MUW66_RS10585 (MUW66_10585) 2253745..2255001 - 1257 WP_003902247.1 HNH endonuclease signature motif containing protein -
MUW66_RS10590 (MUW66_10590) 2255355..2255555 + 201 Protein_2093 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T241046 WP_003410075.1 NZ_CP095021:2250202-2250600 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T241046 NZ_LT906456:2430435-2430806 [Actinobacillus suis]
ATGTATGAAATAGTCTTTTATCGAGACCGACGTGGGCGAGAACCCGTCAAGGAATTCCTGCAGGAATTTATTAATGAACA
ACAAGACGAAAATCGAGAGCGTTTGCACAAAATTAGTCATCATTTGACGATTTTACATTTGCACGGCACTCGAGCAGGCG
AAAGTTATATTAAGCATTTAGAAGATCGTATTTGGCAGTTACGTCCAATTAGCGATTGTTTGCTCTTCGCCGGTATTGTA
CGAGGTCAGTTTGTATTGTTGCATCATTTCGCCAAAAGCAGTAGTCGGCTTCCTAAGCGTGAGCTTGAGCGAGCAAAAAG
TCGTTTGGCAGATCTACAGGAGAGGATAAAAGATGAGCCCCATTGGTTCTAG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8346.16 Da        Isoelectric Point: 4.1621

>AT241046 WP_003911750.1 NZ_CP095021:2249974-2250201 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 228 bp

>AT241046 NZ_LT906456:2430787-2431077 [Actinobacillus suis]
ATGAGCCCCATTGGTTCTAGTTGGACATCATTTGAACGAGAAGTGTTTAATGAGTTGGAAATGGAAGAAAACCATTTTCG
TGTTAAATTGATTCTTGAAATTATTGAGACCCGACAGGCGTTAGGTATTTCACAACGTAAATTAGAAAAACTAAGTGGCG
TAAAACAATCGATGATAGCCCGGGTTGAAAAGGGAAGCTCCAATCCTTCTCTTGGTACGTTACTAAAATTACTGGTGCCG
CTGGGGAAAACATTACAAATCGTGCCTTTAGACGATACTCATTCTAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
AlphaFold DB A0A806JLL8

References