Detailed information of TA system TA241040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2182712..2183415
• Replicon: chromosome
• Accession: NZ_CP095021
• Organism: Mycobacterium tuberculosis strain SGF0422017

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: MUW66_RS10140
• Protein ID: WP_003409778.1
• Coordinates: 2182712..2183041 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: MUW66_RS10145
• Protein ID: WP_003409780.1
• Coordinates: 2183038..2183415 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW66_RS10120 (MUW66_10120)	2179095..2180165	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
MUW66_RS10125 (MUW66_10125)	2180162..2180677	+	516	WP_003409718.1	flavin reductase family protein	-
MUW66_RS10130 (MUW66_10130)	2180674..2181735	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
MUW66_RS10135 (MUW66_10135)	2181732..2182502	+	771	WP_003409775.1	SDR family oxidoreductase	-
MUW66_RS10140 (MUW66_10140)	2182712..2183041	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUW66_RS10145 (MUW66_10145)	2183038..2183415	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
MUW66_RS10150 (MUW66_10150)	2183412..2184002	-	591	WP_003409784.1	SEC-C metal-binding domain-containing protein	-
MUW66_RS10155 (MUW66_10155)	2184057..2185421	+	1365	WP_003903691.1	HNH endonuclease signature motif containing protein	-
MUW66_RS10160 (MUW66_10160)	2185576..2186028	-	453	WP_003899095.1	lipoprotein	-
MUW66_RS10165 (MUW66_10165)	2186092..2186493	+	402	WP_003409869.1	hypothetical protein	-
MUW66_RS10170 (MUW66_10170)	2186486..2186668	-	183	WP_003409870.1	hypothetical protein	-
MUW66_RS10175 (MUW66_10175)	2186782..2187132	-	351	WP_003409871.1	hypothetical protein	-
MUW66_RS10180 (MUW66_10180)	2187143..2188045	-	903	WP_003899097.1	hypothetical protein	-
MUW66_RS10185 (MUW66_10185)	2188066..2188257	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T241040 WP_003409778.1 NZ_CP095021:c2183041-2182712 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T241040 NZ_LT906455:1580271-1580495 [Aliarcobacter butzleri]
ATGAGTAAAAAAGATAAACTATTAATAAAATTTTTGGAAATTCCTCCAAGGAAAGATTTAACTTTTAAAGAACTAAGTAC
TTTACTTATATCTTTAGGTTTTGAGAAAATCGAAGGAGCTGGTTCTGCTGTAAAGTTTTACAATAAAGAAAAAGATTTAT
TAATAAATCTTCATAAACCACATCCAAGTGATATTTTAAAAGTTTATCTAATAAAACAAATTTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT241040 WP_003409780.1 NZ_CP095021:c2183415-2183038 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT241040 NZ_LT906455:1580519-1580863 [Aliarcobacter butzleri]
ATGTCAAATATCATCGAATATAACGGATATATAGGTACTATAGAGTATTCTCAAGAGGATAAATGTTTTTTTGGAAAAAT
TGATATGATAAATGATTTAATAACATTTGAAGCCCAAAATGCTACAGAACTGGAAGAAAATTTTAAAAATGCAGTTGATG
AATATGTACAAACATGTAAACAACTAAATAGAGAACCACAAAAAGCATTTAAAGGTGTTTTTAATGTACGAACAGGAAGT
GAACTTCATAGACTCGCTGTATTAAATGCTACTAAAATTGGGGTTTCTTTGAATACTTATATCAAAAGTTTAATAGAAAA
AGATAATAAACTATCTTTAAATTAG

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8