Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1769422..1770035 Replicon chromosome
Accession NZ_CP095021
Organism Mycobacterium tuberculosis strain SGF0422017

Toxin (Protein)


Gene name vapC Uniprot ID P64880
Locus tag MUW66_RS08295 Protein ID WP_003407786.1
Coordinates 1769631..1770035 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8UJ46
Locus tag MUW66_RS08290 Protein ID WP_009935474.1
Coordinates 1769422..1769625 (+) Length 68 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW66_RS08260 (MUW66_08260) 1764828..1765208 + 381 WP_003407768.1 fumarate reductase subunit C -
MUW66_RS08265 (MUW66_08265) 1765205..1765582 + 378 WP_003407771.1 fumarate reductase subunit FrdD -
MUW66_RS08270 (MUW66_08270) 1765650..1766258 + 609 WP_003407773.1 TetR/AcrR family transcriptional regulator -
MUW66_RS08275 (MUW66_08275) 1766441..1767589 + 1149 Protein_1631 MMPL family transporter -
MUW66_RS08280 (MUW66_08280) 1767599..1768045 + 447 WP_003407780.1 F420H(2)-dependent quinone reductase -
MUW66_RS08285 (MUW66_08285) 1768080..1769369 + 1290 WP_003407781.1 threonine ammonia-lyase -
MUW66_RS08290 (MUW66_08290) 1769422..1769625 + 204 WP_009935474.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MUW66_RS08295 (MUW66_08295) 1769631..1770035 + 405 WP_003407786.1 type II toxin-antitoxin system toxin ribonuclease VapC11 Toxin
MUW66_RS08300 (MUW66_08300) 1770052..1771794 - 1743 WP_003407788.1 malto-oligosyltrehalose trehalohydrolase -
MUW66_RS08305 (MUW66_08305) 1771787..1774084 - 2298 WP_003407790.1 malto-oligosyltrehalose synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14650.76 Da        Isoelectric Point: 5.6587

>T241037 WP_003407786.1 NZ_CP095021:1769631-1770035 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Download         Length: 405 bp

>T241037 NZ_LT906454:c316011-315676 [Streptococcus acidominimus]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATATTTTAGAGAAGTTAAAAGAAATTCATGATTATATTTCTCA
AAACTATTCCTCAATATCAGCTCAGCATAAAATGGAGCAAATCATTAGTGATATAGAAAAACTCGAGGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCAGCAAATATCACAGTACTAGAGGTTATACCTTAAGTAAGGAT
TATATTGTCTTATACCACATTGAGGAGGAAGAAGGCAGAGTTGTTATTGATTACTTACTTCCTACTCGAAGCGACTACAT
GAAATTATTCAAATAG

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7501.48 Da        Isoelectric Point: 4.3951

>AT241037 WP_009935474.1 NZ_CP095021:1769422-1769625 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Download         Length: 204 bp

>AT241037 NZ_LT906454:c316288-316001 [Streptococcus acidominimus]
ATGGTTACAGCAGAAAAAAAAAGAGCTGTCACATTCCAAGCTAACAAAGAATTGGTAAGCGAAGCAATGACGGTATTAAA
CAAAAAAAATTTGACCTTATCATCTGCCTTGAGATTATTTCTACAAAATGTCGTTGTCACAAATGAGGTTGACTTATTGA
CAGAAGAGGAGCTAGAAAAAGAAAAACTTTTCAAGCAATTTCAAGCTGAAATCAATAAAAATATTGAGGACGTTCGTCAA
GGTAAGTTTTATACCTCCGAAGAAGTGAGGACTGAACTTGGACTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6A7V

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8UJ46

References