Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1690924..1691540 Replicon chromosome
Accession NZ_CP095021
Organism Mycobacterium tuberculosis strain SGF0422017

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag MUW66_RS07950 Protein ID WP_003407593.1
Coordinates 1691223..1691540 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag MUW66_RS07945 Protein ID WP_003900349.1
Coordinates 1690924..1691226 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW66_RS07935 (MUW66_07935) 1686810..1688657 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
MUW66_RS07940 (MUW66_07940) 1688658..1690910 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
MUW66_RS07945 (MUW66_07945) 1690924..1691226 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
MUW66_RS07950 (MUW66_07950) 1691223..1691540 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
MUW66_RS07955 (MUW66_07955) 1691537..1692541 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
MUW66_RS07960 (MUW66_07960) 1692594..1693883 + 1290 WP_003902090.1 serine hydrolase domain-containing protein -
MUW66_RS07965 (MUW66_07965) 1693956..1694681 - 726 WP_003898898.1 class I SAM-dependent methyltransferase -
MUW66_RS07970 (MUW66_07970) 1694787..1694999 - 213 WP_003898900.1 dodecin family protein -
MUW66_RS07975 (MUW66_07975) 1695060..1695458 + 399 WP_003900351.1 hypothetical protein -
MUW66_RS07980 (MUW66_07980) 1695503..1696531 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T241036 WP_003407593.1 NZ_CP095021:1691223-1691540 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T241036 NZ_LT906452:c3411004-3410708 [Legionella pneumophila]
ATGATTTATGAGCTTCATTTTCATCCTTTAGCTTTAAAAGAATGGAAAAAGCTGGGGAAAAACCTTCAAGAAGAATTTAA
GAAAGTGTTAAAACGCCGTTTAGAAAATCCACATGTGGCTTCGGCTGGCTTAAGAGGCTCTTTAAAAAATTGCTATAAAA
TAAAATTACGTCAATCTGGTTATCGTTTGGTGTATCAAGTTAATGATAATAAATTAATTGTAACCGTCATAGCAGTCGGG
AAAAGAAACAAAAATGTTGTCTATGATGATGCAGATAACCGACAAGAAGACAGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT241036 WP_003900349.1 NZ_CP095021:1690924-1691226 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT241036 NZ_LT906452:c3411245-3410994 [Legionella pneumophila]
ATGCAAACTTTATTTGCTACACAAGTAGCGAGCATTAGTGAATTAAAAAAGAACCCCACTAAGCTCATTAATGATGCTCA
TGGAAAACCTATTGCTATTCTTAACCACAATACAGCGGCAGCATATTTAATTCCTGTTGAAACATTTGAAAAACTAATGG
ATTTGATTGATGAGAATGAACTACATAAAATAGTAGAAGAGCGTTTGTCAGCACCTTTTACCTCAATAAAGGTAAATATT
GATGATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References