Detailed information of TA system TA241031

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239419..1239987
• Replicon: chromosome
• Accession: NZ_CP095021
• Organism: Mycobacterium tuberculosis strain SGF0422017

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: MUW66_RS05935
• Protein ID: WP_003405865.1
• Coordinates: 1239613..1239987 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: MUW66_RS05930
• Protein ID: WP_003405863.1
• Coordinates: 1239419..1239616 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW66_RS05910 (MUW66_05910)	1235460..1236098	-	639	WP_003898730.1	lipid droplet-associated protein	-
MUW66_RS05915 (MUW66_05915)	1236188..1237195	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
MUW66_RS05920 (MUW66_05920)	1237212..1238195	-	984	WP_003898731.1	hypothetical protein	-
MUW66_RS05925 (MUW66_05925)	1238258..1239331	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
MUW66_RS05930 (MUW66_05930)	1239419..1239616	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MUW66_RS05935 (MUW66_05935)	1239613..1239987	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUW66_RS05940 (MUW66_05940)	1240190..1240888	+	699	WP_003898733.1	hypothetical protein	-
MUW66_RS05945 (MUW66_05945)	1241006..1241191	+	186	WP_003901093.1	hypothetical protein	-
MUW66_RS05950 (MUW66_05950)	1241118..1241393	-	276	WP_003405867.1	hypothetical protein	-
MUW66_RS05955 (MUW66_05955)	1241636..1241959	+	324	WP_003405871.1	putative quinol monooxygenase	-
MUW66_RS05960 (MUW66_05960)	1241974..1242672	-	699	WP_031646953.1	hypothetical protein	-
MUW66_RS05965 (MUW66_05965)	1242706..1242915	+	210	WP_003911400.1	hypothetical protein	-
MUW66_RS05970 (MUW66_05970)	1242867..1243637	-	771	Protein_1175	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T241031 WP_003405865.1 NZ_CP095021:1239613-1239987 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T241031 NZ_LT906447:1042354-1042704 [Staphylococcus piscifermentans]
ATGATGAGGAGAGGCGATGTTTACTTAGCTGATTTATCACCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGACCTGTTGT
AATTATACAAAATGACACTGGAAATAAGTATAGCCCGACTGTTATTGTAGCTGCAATAACGGGTCGGATTAATAAAGCGA
AAATTCCAACTCATGTTGAAATCGAGAAAAGTAAATACAAACTGGACAAAGATTCAGTTATTTTACTTGAACAAATTAGA
ACAGTCGACAAGAAACGTCTAAAGGAAAAACTGACATATTTATCAGACGAAAAGATGAAAGAAATTGATAATGCGATTCA
AATCAGCTTAGGCTTGACACATCGCAATTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT241031 WP_003405863.1 NZ_CP095021:1239419-1239616 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT241031 NZ_LT906447:1042190-1042357 [Staphylococcus piscifermentans]
ATGTCAACTTTTAATCAGAATAGAAGTTATAGTTTAGAACAGTCACTGAAAGAAGGCTACGCACAGATGGCTGACTTAAA
TCTCTCCCTCGCAACGGAGGCATTTTCAGTAGAATGTGAAGCCTGTGATTGCAACGAATCATACCTAGCTTTCGACAAGG
ATGAATGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07