Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapC-mazE/PIN(toxin)
Location 753893..754685 Replicon chromosome
Accession NZ_CP095021
Organism Mycobacterium tuberculosis strain SGF0422017

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB2
Locus tag MUW66_RS03475 Protein ID WP_003403386.1
Coordinates 754248..754685 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag MUW66_RS03470 Protein ID WP_003403381.1
Coordinates 753893..754138 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW66_RS03440 (MUW66_03440) 748913..750418 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
MUW66_RS03445 (MUW66_03445) 750499..751509 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
MUW66_RS03450 (MUW66_03450) 751897..752280 - 384 WP_003403365.1 ribonuclease VapC6 -
MUW66_RS03455 (MUW66_03455) 752375..752530 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
MUW66_RS03460 (MUW66_03460) 752606..753322 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
MUW66_RS03465 (MUW66_03465) 753598..753906 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
MUW66_RS03470 (MUW66_03470) 753893..754138 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
MUW66_RS03475 (MUW66_03475) 754248..754685 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin Toxin
MUW66_RS03480 (MUW66_03480) 754682..754936 - 255 WP_003911263.1 antitoxin VapB7 -
MUW66_RS03485 (MUW66_03485) 755050..757413 + 2364 WP_003901895.1 arylsulfatase AtsD -
MUW66_RS03490 (MUW66_03490) 757476..757802 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MUW66_RS03495 (MUW66_03495) 757714..758052 + 339 WP_003403405.1 PIN domain-containing protein -
MUW66_RS03500 (MUW66_03500) 758049..758222 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T241025 WP_003403386.1 NZ_CP095021:c754685-754248 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T241025 NZ_LT906442:2454283-2454579 [Legionella waltersii]
ATGTCAAATAAAACATATCGTCTTTACCCAAAAGCGGTAGAAGATCTTGAGTCCATCTATTTATATAGCACAAATGAGTT
TGGTATCAAACAAACAGAGGATTATATTTTAACCATAGATTTAAGTTTTCAGCGCCTTGCAGATGATCCCGTAATTGCTC
GAAAATGTGATTATATTCGTCCAGAATTACACGCATTTAATGTTGGCTCTCACATTATTTTTTTCAAAACTACCGATTAT
GGCATTTCTGTTATTCGAGTGTTGCATCAATCTATGGATTTTAATAGACATCTATAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT241025 WP_003403381.1 NZ_CP095021:c754138-753893 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT241025 NZ_LT906442:2454039-2454293 [Legionella waltersii]
ATGACCCGTACAATGACTGTTGATTTGGGTAATGAACTTCGTAATTACGTGGAATCACTTGTGCGCTCAGGGGATTATAG
AAGTAATAGCGAAGTATTAAGAGAGTCATTGCGTTTACTTCGTGAAAAACAAGCTGAATCAAAACTAGAGCAATTGCGTC
ACCTTATAGATGAAGGAGAAGCTAGCGGGAAGCCTGAAACATGGGATGCACATGATTTTCTTAAGAAAATGAAGAAAAAA
TCCCATGTCAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSW5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References