Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 714790..715439 Replicon chromosome
Accession NZ_CP095021
Organism Mycobacterium tuberculosis strain SGF0422017

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag MUW66_RS03265 Protein ID WP_003403236.1
Coordinates 715044..715439 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag MUW66_RS03260 Protein ID WP_003403235.1
Coordinates 714790..715044 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW66_RS03235 (MUW66_03235) 709916..709999 + 84 Protein_641 galactose-1-phosphate uridylyltransferase -
MUW66_RS03240 (MUW66_03240) 710018..711099 + 1082 Protein_642 galactose-1-phosphate uridylyltransferase -
MUW66_RS03245 (MUW66_03245) 711096..712187 + 1092 WP_003900977.1 galactokinase -
MUW66_RS03250 (MUW66_03250) 712582..713646 + 1065 WP_003898532.1 zinc ribbon domain-containing protein -
MUW66_RS03255 (MUW66_03255) 713750..714697 + 948 WP_003403232.1 DUF732 domain-containing protein -
MUW66_RS03260 (MUW66_03260) 714790..715044 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
MUW66_RS03265 (MUW66_03265) 715044..715439 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
MUW66_RS03270 (MUW66_03270) 715533..716273 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
MUW66_RS03275 (MUW66_03275) 716405..716665 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MUW66_RS03280 (MUW66_03280) 716662..717069 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
MUW66_RS03285 (MUW66_03285) 717141..718292 - 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
MUW66_RS03290 (MUW66_03290) 718385..720112 - 1728 WP_003901883.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T241021 WP_003403236.1 NZ_CP095021:715044-715439 [Mycobacterium tuberculosis]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T241021 NZ_LT906438:528925-529275 [Bacillus pumilus]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTTAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACAATTGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT241021 WP_003403235.1 NZ_CP095021:714790-715044 [Mycobacterium tuberculosis]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT241021 NZ_LT906438:528639-528920 [Bacillus pumilus]
TTGTCTGAATCCAGCGCAACAAATGAAATGAAGATTCGACTGCCGGAGGCTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAGTTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References