Detailed information of TA system TA241019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 701624..702267
• Replicon: chromosome
• Accession: NZ_CP095021
• Organism: Mycobacterium tuberculosis strain SGF0422017

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: MUW66_RS03180
• Protein ID: WP_003403187.1
• Coordinates: 701866..702267 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: MUW66_RS03175
• Protein ID: WP_003403184.1
• Coordinates: 701624..701869 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW66_RS03140 (MUW66_03140)	696904..697434	-	531	WP_003917320.1	two component system sensor kinase HK2	-
MUW66_RS03145 (MUW66_03145)	697418..698113	-	696	WP_003918099.1	two-component system response regulator TcrA	-
MUW66_RS03150 (MUW66_03150)	698236..698547	+	312	WP_003403164.1	hypothetical protein	-
MUW66_RS03155 (MUW66_03155)	698619..699569	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
MUW66_RS03160 (MUW66_03160)	699810..700394	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
MUW66_RS03165 (MUW66_03165)	700396..701106	+	711	Protein_627	IS607 family element RNA-guided endonuclease TnpB	-
MUW66_RS03170 (MUW66_03170)	701109..701579	+	471	WP_003898523.1	hypothetical protein	-
MUW66_RS03175 (MUW66_03175)	701624..701869	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MUW66_RS03180 (MUW66_03180)	701866..702267	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUW66_RS03185 (MUW66_03185)	702258..702437	+	180	Protein_631	hypothetical protein	-
MUW66_RS03190 (MUW66_03190)	702855..703052	-	198	WP_003403191.1	hypothetical protein	-
MUW66_RS03195 (MUW66_03195)	703132..704289	-	1158	WP_003898524.1	hypothetical protein	-
MUW66_RS03200 (MUW66_03200)	704341..704562	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
MUW66_RS03205 (MUW66_03205)	704704..705309	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T241019 WP_003403187.1 NZ_CP095021:701866-702267 [Mycobacterium tuberculosis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T241019 NZ_LT906437:c961631-961212 [Neisseria gonorrhoeae]
ATGATTTTGCTGGACACGAATGTGATTTCCGAACCTTTGCGCCCACAACCCAATGAACGTGTGGTGGCATGGTTGGATAG
TTTGATATTGGAAGATGTGTATTTGTCTGCCATTACTGTTGCAGAATTGCGTTTGGGTGTGGCGTTGTTGCTCAATGGCA
AGAAAAAGAATGTGCTGCACGAACGTTTGGAACAATCCATTTTGCCTTTATTTGCGGGGCGGATTCTGCCTTTTGATGAA
CCGGTTGCCGCAATCTATGCGCAAATTCGTTCCTATGCCAAAACACATGGCAAAGAGATTGCTGCCGCAGACGGCTATAT
TGCCGCCACTGCAAAACAGCACAGTTTGACAGTTGCTACGCGTGATACCGGCTCATTTTTTGCGGCCGATGTCGCGGTGT
TCAATCCGTGGCACGATTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT241019 WP_003403184.1 NZ_CP095021:701624-701869 [Mycobacterium tuberculosis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT241019 NZ_LT906437:c961867-961631 [Neisseria gonorrhoeae]
ATGGCTTCTGTTGTGATTAGAAATTTATCCGAGGCCACGCACAACGCAATCAAATTCCGTGCGCGAGCCGCAGGGCGCAG
TACCGAAGCAGAAATCCGCTTAATTTTGGATAACATCGCCAAAGCACAACAAACTGTACGTTTGGGGTCAATGTTGGCAT
CAATAGGGCAGGAAATCGGAGGTGTTGAGCTGGAAGACGTACGCGGTCGTAATACTGATAACGAGGTTTCTTTGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91