Detailed information of TA system TA241015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 638613..639289
• Replicon: chromosome
• Accession: NZ_CP095021
• Organism: Mycobacterium tuberculosis strain SGF0422017

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: MUW66_RS02875
• Protein ID: WP_003898500.1
• Coordinates: 638613..639026 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: MUW66_RS02880
• Protein ID: WP_003402915.1
• Coordinates: 639023..639289 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW66_RS02845 (MUW66_02845)	633958..634260	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
MUW66_RS02850 (MUW66_02850)	634320..634598	-	279	WP_003402901.1	hypothetical protein	-
MUW66_RS02855 (MUW66_02855)	634595..635848	-	1254	WP_003402904.1	inorganic phosphate transporter	-
MUW66_RS02860 (MUW66_02860)	635968..636354	-	387	WP_003402907.1	VOC family protein	-
MUW66_RS02865 (MUW66_02865)	636417..637301	-	885	WP_003402909.1	SDR family oxidoreductase	-
MUW66_RS02870 (MUW66_02870)	637397..638299	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
MUW66_RS02875 (MUW66_02875)	638613..639026	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUW66_RS02880 (MUW66_02880)	639023..639289	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
MUW66_RS02885 (MUW66_02885)	639481..641196	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
MUW66_RS02890 (MUW66_02890)	641274..642878	+	1605	WP_003402920.1	amidohydrolase	-
MUW66_RS02895 (MUW66_02895)	642875..643855	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T241015 WP_003898500.1 NZ_CP095021:c639026-638613 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T241015 NZ_LT906435:c713563-713261 [Pandoraea sputorum]
GTGAAATTGGAGTGGTCCCAACATGCAATTCAAGACCGTAGCGACATTTATGACTTTGTTGAGCAACGCAGTCCCAACGC
GGCGATCTGGATCGACTCCCGAATCAACCAGCAACTTCTCTCACTGTTACGTTTCCCGCGAGCGGGACGACCCGGAAGTA
TCCGTGGAACCCGAGAACTTGTAATCACGCGAACGCCTTATATCGCCGCGTATCGCGCGCTACCCACTCGCATTCGAGTC
CTCCGCATTTTCCACAACGCCCAAATGTGGCCAGGCGACGCACCTACCGGGCCGCACCATTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT241015 WP_003402915.1 NZ_CP095021:c639289-639023 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT241015 NZ_LT906435:c713872-713567 [Pandoraea sputorum]
ATGGCAGCAAGTGCACTGGTGCAGGCACGTATCGATCCGGCGCTAAAAGAGCGCGCCTCGGCGGTACTCGACAAGATGGG
TTTGACGGTCTCGGATGTAGTTCGGATTTTACTCACACGTATTGCGAACGAAGGTGCGCTACCGTTTGGCTTCGCTGCGG
ATCCGGACGCACACGATGCATGGGTCAGACAGAAAGTGCTGGAAGCGCTCGAAGATACGCGTCCGGCAATCGCTCACGAT
GACGTCGAATCACACTTCGCTGCTCGTCGCGAAGCAACGCGAAAACGCATATCGAAAAAAAGGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0