Detailed information of TA system TA241004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3171768..3172455
• Replicon: chromosome
• Accession: NZ_CP095020
• Organism: Mycobacterium tuberculosis strain SGF0232017

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: MUW65_RS15135
• Protein ID: WP_003414624.1
• Coordinates: 3172012..3172455 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: MUW65_RS15130
• Protein ID: WP_003414620.1
• Coordinates: 3171768..3172025 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW65_RS15110 (MUW65_15110)	3167088..3167942	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
MUW65_RS15115 (MUW65_15115)	3167998..3169161	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
MUW65_RS15120 (MUW65_15120)	3169178..3170392	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
MUW65_RS15125 (MUW65_15125)	3170400..3171641	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
MUW65_RS15130 (MUW65_15130)	3171768..3172025	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
MUW65_RS15135 (MUW65_15135)	3172012..3172455	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUW65_RS15140 (MUW65_15140)	3172535..3173197	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
MUW65_RS15145 (MUW65_15145)	3173293..3173484	+	192	WP_003414632.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
MUW65_RS15150 (MUW65_15150)	3173816..3175564	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
MUW65_RS15155 (MUW65_15155)	3175660..3176241	+	582	WP_003414644.1	fasciclin domain-containing protein	-
MUW65_RS15160 (MUW65_15160)	3176341..3176607	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T241004 WP_003414624.1 NZ_CP095020:3172012-3172455 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T241004 NZ_LT905060:c3919821-3919534 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGTAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT241004 WP_003414620.1 NZ_CP095020:3171768-3172025 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT241004 NZ_LT905060:c3919547-3919245 [Salmonella enterica subsp. enterica serovar Typhi]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGACAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGACGTTATGGAATGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTGAATGCCATTCTGCGCGAAGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• PDB: 7VWO

Antitoxin

• PDB: 7VWO
• AlphaFold DB: G0TFH0