Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2802720..2803372 Replicon chromosome
Accession NZ_CP095020
Organism Mycobacterium tuberculosis strain SGF0232017

Toxin (Protein)


Gene name vapC Uniprot ID A0A5R1ZCY8
Locus tag MUW65_RS13150 Protein ID WP_003905869.1
Coordinates 2802947..2803372 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ24
Locus tag MUW65_RS13145 Protein ID WP_003412749.1
Coordinates 2802720..2802941 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW65_RS13130 (MUW65_13130) 2800960..2801166 - 207 WP_003899347.1 hypothetical protein -
MUW65_RS13135 (MUW65_13135) 2801302..2801925 + 624 WP_003900858.1 TIGR00725 family protein -
MUW65_RS13140 (MUW65_13140) 2801915..2802667 + 753 WP_003901416.1 hypothetical protein -
MUW65_RS13145 (MUW65_13145) 2802720..2802941 + 222 WP_003412749.1 antitoxin Antitoxin
MUW65_RS13150 (MUW65_13150) 2802947..2803372 + 426 WP_003905869.1 type II toxin-antitoxin system VapC family toxin Toxin
MUW65_RS13155 (MUW65_13155) 2803395..2804576 - 1182 WP_003905870.1 dihydrolipoamide acetyltransferase family protein -
MUW65_RS13160 (MUW65_13160) 2804573..2805619 - 1047 WP_003412757.1 3-methyl-2-oxobutanoate dehydrogenase subunit beta -
MUW65_RS13165 (MUW65_13165) 2805630..2806733 - 1104 WP_003412761.1 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha -
MUW65_RS13170 (MUW65_13170) 2806992..2807813 - 822 WP_003412766.1 citrate (pro-3S)-lyase subunit beta -
MUW65_RS13175 (MUW65_13175) 2807810..2808367 - 558 WP_003412768.1 MaoC family dehydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15364.84 Da        Isoelectric Point: 9.2386

>T240991 WP_003905869.1 NZ_CP095020:2802947-2803372 [Mycobacterium tuberculosis]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRASTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Download         Length: 426 bp

>T240991 NZ_LT903847:3199605-3199883 [Escherichia coli O127:H6]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 7864.93 Da        Isoelectric Point: 4.5258

>AT240991 WP_003412749.1 NZ_CP095020:2802720-2802941 [Mycobacterium tuberculosis]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Download         Length: 222 bp

>AT240991 NZ_LT903847:3199883-3200167 [Escherichia coli O127:H6]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCTGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5R1ZCY8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW03

References