Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2319574..2320210 Replicon chromosome
Accession NZ_CP095020
Organism Mycobacterium tuberculosis strain SGF0232017

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag MUW65_RS10845 Protein ID WP_003410654.1
Coordinates 2319800..2320210 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag MUW65_RS10840 Protein ID WP_003410651.1
Coordinates 2319574..2319807 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW65_RS10825 (MUW65_10825) 2315022..2315423 + 402 WP_009937711.1 metal ABC transporter permease -
MUW65_RS10830 (MUW65_10830) 2315424..2315828 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
MUW65_RS10835 (MUW65_10835) 2315912..2319496 - 3585 WP_003910889.1 cobaltochelatase subunit CobN -
MUW65_RS10840 (MUW65_10840) 2319574..2319807 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
MUW65_RS10845 (MUW65_10845) 2319800..2320210 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
MUW65_RS10850 (MUW65_10850) 2320194..2321285 + 1092 WP_003900454.1 precorrin-3B synthase -
MUW65_RS10855 (MUW65_10855) 2321295..2321921 + 627 WP_003410658.1 precorrin-8X methylmutase -
MUW65_RS10860 (MUW65_10860) 2321918..2323444 + 1527 WP_244702837.1 precorrin-2 C(20)-methyltransferase -
MUW65_RS10865 (MUW65_10865) 2323390..2324613 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T240988 WP_003410654.1 NZ_CP095020:2319800-2320210 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T240988 NZ_LT903847:2654273-2654527 [Escherichia coli O127:H6]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT240988 WP_003410651.1 NZ_CP095020:2319574-2319807 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT240988 NZ_LT903847:2654025-2654276 [Escherichia coli O127:H6]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References