Detailed information of TA system TA240986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2250431..2251057
• Replicon: chromosome
• Accession: NZ_CP095020
• Organism: Mycobacterium tuberculosis strain SGF0232017

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: MUW65_RS10545
• Protein ID: WP_003410075.1
• Coordinates: 2250659..2251057 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: MUW65_RS10540
• Protein ID: WP_003911750.1
• Coordinates: 2250431..2250658 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW65_RS10530 (MUW65_10530)	2248470..2248814	-	345	WP_003410065.1	ferredoxin family protein	-
MUW65_RS10535 (MUW65_10535)	2249003..2250172	-	1170	WP_003899126.1	ATP-binding protein	-
MUW65_RS10540 (MUW65_10540)	2250431..2250658	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MUW65_RS10545 (MUW65_10545)	2250659..2251057	+	399	WP_003410075.1	PIN domain nuclease	Toxin
MUW65_RS10550 (MUW65_10550)	2251240..2251671	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
MUW65_RS10555 (MUW65_10555)	2251772..2252206	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
MUW65_RS10560 (MUW65_10560)	2252643..2252822	-	180	Protein_2087	hypothetical protein	-
MUW65_RS10565 (MUW65_10565)	2252910..2253530	+	621	WP_024753640.1	IS110 family transposase	-
MUW65_RS10570 (MUW65_10570)	2253484..2254074	+	591	WP_003899131.1	IS110 family transposase	-
MUW65_RS10575 (MUW65_10575)	2254202..2255458	-	1257	WP_003902247.1	HNH endonuclease signature motif containing protein	-
MUW65_RS10580 (MUW65_10580)	2255812..2256012	+	201	Protein_2091	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T240986 WP_003410075.1 NZ_CP095020:2250659-2251057 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T240986 NZ_LT903847:1793904-1794215 [Escherichia coli O127:H6]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT240986 WP_003911750.1 NZ_CP095020:2250431-2250658 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT240986 NZ_LT903847:1794212-1794631 [Escherichia coli O127:H6]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAATAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGTCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTATCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8