Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2217800..2218488 Replicon chromosome
Accession NZ_CP095020
Organism Mycobacterium tuberculosis strain SGF0232017

Toxin (Protein)


Gene name vapC Uniprot ID P0A653
Locus tag MUW65_RS10375 Protein ID WP_003409958.1
Coordinates 2217800..2218219 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag MUW65_RS10380 Protein ID WP_003409968.1
Coordinates 2218228..2218488 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW65_RS10355 (MUW65_10355) 2213295..2214143 + 849 WP_003901306.1 class I SAM-dependent methyltransferase -
MUW65_RS10360 (MUW65_10360) 2214106..2215551 - 1446 WP_003899115.1 APC family permease -
MUW65_RS10365 (MUW65_10365) 2215730..2216416 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
MUW65_RS10370 (MUW65_10370) 2216607..2217575 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
MUW65_RS10375 (MUW65_10375) 2217800..2218219 - 420 WP_003409958.1 type II toxin-antitoxin system VapC family toxin Toxin
MUW65_RS10380 (MUW65_10380) 2218228..2218488 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MUW65_RS10385 (MUW65_10385) 2218631..2220307 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
MUW65_RS10390 (MUW65_10390) 2220295..2220948 - 654 WP_244702835.1 cutinase Cfp21 -
MUW65_RS10395 (MUW65_10395) 2221063..2221293 + 231 WP_003409980.1 DUF167 domain-containing protein -
MUW65_RS10400 (MUW65_10400) 2221378..2222289 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
MUW65_RS10405 (MUW65_10405) 2222398..2222997 + 600 WP_003910883.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14724.89 Da        Isoelectric Point: 7.9535

>T240983 WP_003409958.1 NZ_CP095020:c2218219-2217800 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Download         Length: 420 bp

>T240983 NZ_LT903847:1090786-1091061 [Escherichia coli O127:H6]
ATGAGAACCCAGGTACAGGCTTTGCGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT240983 WP_003409968.1 NZ_CP095020:c2218488-2218228 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT240983 NZ_LT903847:1091058-1091417 [Escherichia coli O127:H6]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C4H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References