Detailed information of TA system TA240977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1768116..1768729
• Replicon: chromosome
• Accession: NZ_CP095020
• Organism: Mycobacterium tuberculosis strain SGF0232017

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: MUW65_RS08295
• Protein ID: WP_003407786.1
• Coordinates: 1768325..1768729 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: MUW65_RS08290
• Protein ID: WP_009935474.1
• Coordinates: 1768116..1768319 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW65_RS08260 (MUW65_08260)	1763521..1763901	+	381	WP_003407768.1	fumarate reductase subunit C	-
MUW65_RS08265 (MUW65_08265)	1763898..1764275	+	378	WP_003407771.1	fumarate reductase subunit FrdD	-
MUW65_RS08270 (MUW65_08270)	1764343..1764951	+	609	WP_003407773.1	TetR/AcrR family transcriptional regulator	-
MUW65_RS08275 (MUW65_08275)	1765135..1766283	+	1149	Protein_1631	MMPL family transporter	-
MUW65_RS08280 (MUW65_08280)	1766293..1766739	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
MUW65_RS08285 (MUW65_08285)	1766774..1768063	+	1290	WP_003407781.1	threonine ammonia-lyase	-
MUW65_RS08290 (MUW65_08290)	1768116..1768319	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MUW65_RS08295 (MUW65_08295)	1768325..1768729	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
MUW65_RS08300 (MUW65_08300)	1768746..1770488	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
MUW65_RS08305 (MUW65_08305)	1770481..1772778	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T240977 WP_003407786.1 NZ_CP095020:1768325-1768729 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T240977 NZ_LT900341:14214-14483 [Gluconobacter oxydans 621H]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT240977 WP_009935474.1 NZ_CP095020:1768116-1768319 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT240977 NZ_LT900341:13942-14217 [Gluconobacter oxydans 621H]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46